Leishmaniasis in cat shelters: A serological, molecular and entomological study

Autor(es)

Franco Leonel, Joao Augusto ; Vioti, Geovanna ; Alves, Maria Luana ; Benassi, Julia Cristina ; da Silva, Diogo Tiago ; Pereira Spada, Julio Cesar ; de Azevedo Ruiz, Vera Letticie ; Starke-Buzetti, Wilma Aparecida ; Soares, Rodrigo Martins ; Ferreira de Sousa Oliveira, Tricia Maria

• Tipo de documento: Artigo Científico
• Fonte: TRANSBOUNDARY AND EMERGING DISEASES; v. 67, n. 5, p. 7-pg., 2020-03-24.

Resumo

An epidemiologicalLeishmaniaspp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood and conjunctival swab (CS) samples were collected from 94 cats in two animal protection shelters. These samples were subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) and indirect enzyme-linked immunosorbent assay (ELISA) and to molecular test by polymerase chain reaction (PCR). In addition, a Phlebotomine sandflies survey was performed in the same shelters. The analyses revealed a positivity of 31.91% (30/94) through ELISA and 29.79% (28/94) through IFAT. The two serological tests showed a positive association with perfect agreement (k = 0.925). None of the cats were positive byLeishmaniaspp. DNA. OneLutzomyia (Lutzomyia) longipalpismale was found in one of the cat shelters. The results and the implications of our findings are discussed below. (AU)

• Processo FAPESP: 14/02882-3 - Detecção molecular de Leishmania spp. por PCR de sangue e suabe conjuntival em cães e gatos do município de Pirassununga, são Paulo, Brasil
• Beneficiário: João Augusto Franco Leonel
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica