Inhibition of FGF receptor impairs primitive endoderm differentiation in bovine embryos

Autor(es)

Santos, Gabriel S. ; Martins, Matheus P. ; Luedke, Felipe E. ; Tanaka, Yuki ; Carreiro, Leticia E. ; Mendes, Camilla Mota ; Goissis, Marcelo Demarchi

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 58, n. 2, p. 9-pg., 2022-11-14.

Resumo

The first cellular differentiation event in the pre-implantation embryo results in the trophectoderm (TE) and the inner cell mass (ICM). A second event occurs in the latter, resulting in the epiblast and the primitive endoderm (PE). This second differentiation is still not fully characterized in bovine development, although it is likely to involve FGF signalling. Thus, in this study, we tested the hypothesis that stimulation or inhibition of the FGF pathway during bovine embryo in vitro culture would only interfere with PE differentiation if maintained until later blastocyst stages. At first, we characterized the expression of PE marker SOX17 at different blastocyst stages. Then, we treated in vitro produced embryos during different windows of time: days 5.0-7.0 (D5-D7), D7-D9, and D5-D9 with 1 mu g/ml FGF4 and 1 mu g/ml heparin or 1 mM FGFR inhibitor, AZD4547. We observed that the SOX17-positive cell number only increases in late-stage blastocysts compared to early stages. Treatment of embryos with FGF4 did not change the number of SOX17-positive cells, while inhibition of FGFR signalling reduced SOX17-positive cells from D5-D7 and completely ablated SOX17 expression when kept until D9. In conclusion, FGFR inhibition repressed PE differentiation in bovine embryos at all time points, although stimulation with FGF4 did not interfere with PE cell numbers. (AU)

• Processo FAPESP: 19/21303-8 - Efeito da inibição dos receptores de fator de crescimento fibroblástico na diferenciação do endoderma primitivo em embriões bovinos produzidos in vitro
• Beneficiário: Gabriel Siqueira dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/22798-0 - Efeito do fator de crescimento de fibroblasto 4 (FGF4) na diferenciação do endoderma primitivo em embriões bovinos
• Beneficiário: Matheus Pasini Martins
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/09576-3 - Estudos sobre mecanismos de diferenciação celular durante o desenvolvimento embrionário inicial em bovinos
• Beneficiário: Marcelo Demarchi Goissis
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores