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Susceptibility of Vegetative Cells and Endospores of Bacillus cereus to Rhamnolipid Biosurfactants and Their Potential Application in Dairy

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Autor(es):
Bertuso, Paula de Camargo ; Marangon, Crisiane Aparecida ; Nitschke, Marcia
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: MICROORGANISMS; v. 10, n. 9, p. 17-pg., 2022-09-01.
Resumo

Bacillus cereus is a Gram-positive, endospore-forming bacterium well-known as a food pathogen that causes great losses in the food industry, especially in dairy. In this study, rhamnolipid (RL) biosurfactants were evaluated as a bio-based alternative for controlling the growth of vegetative cells and endospores of B. cereus. RLs were tested against 14 B. cereus strains isolated from different types of foodstuffs. The antimicrobial activity against vegetative cells and endospores revealed minimal inhibitory concentration (MIC) values of 0.098 mg/mL for almost all strains tested and minimal bactericidal concentration (MBC) varying between 0.098 and >25 mg/mL. The presence of RLs inhibited endospore germination by more than 99%, reducing by 5.5 log the outgrowth of strain 0426. Scanning and transmission electron microscopy confirmed that exposure to RL causes damage to the structure of endospores. When skim milk was utilized as a food model, RL inhibited the growth of vegetative cells and endospores of B. cereus, showing MBC of 3.13 mg/mL for the vegetative cells of strain 0426. The surfactant also reduced bacterial growth in milk at refrigerator temperature. The results suggest that RLs are promising candidates for the development of novel strategies to control B. cereus in the food industry. (AU)

Processo FAPESP: 19/14405-9 - Explorando o potencial de Ramnolipídeos no controle de patógenos alimentares: efeito da força iônica e biopolímeros
Beneficiário:Marcia Nitschke
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 19/22799-7 - Explorando o potencial de nanopartículas de biopolímeros com extratos de frutas para controle de patógenos alimentares
Beneficiário:Crisiane Aparecida Marangon
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 21/09935-9 - Abordagens inovadoras no uso de ramnolipídeos em alimentos: controle de endósporos e interação com sacarose
Beneficiário:Marcia Nitschke
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
Beneficiário:Osvaldo Novais de Oliveira Junior
Modalidade de apoio: Auxílio à Pesquisa - Temático