Insights into the structure and mechanism of action of the anti-candidal lectin Mo-CBP2 and evaluation of its synergistic effect and antibiofilm activity

Autor(es):Mostrar menos -

Neto, Jao Xavier da Silva ; Dias, Lucas Pinheiro ; de Souza, Larissa Alves Lopes ; da Costa, Helen Paula Silva ; Vasconcelos, Ilka Maria ; Pereira, Mirella Leite ; de Oliveira, Jose Tadeu Abreu ; Cardozo, Claudia Johana Perez ; Moura, Luiz Francisco Wemmenson Gonsalves ; Sousa, Jeanlex Soares de ; Carneiro, Romulo Farias ; Lopes, Tiago Deiveson Pereira ; de Sousa, Daniele de Oliveira Bezerra

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 121, p. 13-pg., 2022-08-17.

Resumo

Plant bioactive compounds are extensively used in traditional medicine to treat diabetes, inflammation, hy-pertension, liver disease, and microbial infections. In this context, plant proteins are promising molecules in the treatment of candidiasis, being an alternative to the classical antifungals currently in use. This study aimed to better understand the structural characteristics of Mo-CBP2, a chitin-binding protein isolated from Moringa oleifera seeds. Its mode of action against Candida spp. was investigated, evaluating protein oligomerization, pore formation, secondary structure, cell wall interaction, and biofilm inhibition. Mo-CBP2 has a trimeric and hex-americ structure, which may explain its aggregation effect against Candida albicans cells. Both yeast aggregation and anti-candida activity were depleted in the presence of N,N,N & PRIME;-acetylchitotriose and laminarin, indicating that Mo-CBP2 interacts with the fungal cell wall. Mo-CBP2 showed high stability at extreme temperatures (100 oC) and pH (2, 4 and 10). Furthermore, treatment with Mo-CBP2 caused pores, severe morphological damage, and the release of cytoplasmic material in Candida. Mo-CBP2 also showed a synergistic effect with azole, polyene, and echinocandin antifungals. Finally, Mo-CBP2 strongly inhibited biomass production in both mature and non -mature biofilms of C. albicans. This study highlights the biotechnological potential of Mo-CBP2 as a promising anti-candida molecule against planktonic and sessile C. albicans cells. (AU)

• Processo FAPESP: 20/03998-6 - Efeito da interação de peptídeos antimicrobianos na estruturação da bicamada lipídica
• Beneficiário: Lucas Pinheiro Dias
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado