Adipose and amnion-derived mesenchymal stem cells: Extracellular vesicles characterization and implication for reproductive biotechnology

Autor(es)

Scassiotti, Rodrigo Ferreira ; Coutinho, Meline de Paula ; Santos, Sarah Ingrid Pinto ; Pinto, Priscilla Avelino Ferreira ; Almeida, Matheus Ferreira ; Karam, Rafael Garcia ; Rosa, Paola Maria da Silva ; Martins, Daniele dos Santos ; da Silveira, Juliano Coelho ; Ambrosio, Carlos Eduardo

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 198, p. 9-pg., 2023-01-07.

Resumo

The stem cell-based research for reproductive biotechnology has been widely studied and shows promise for repairing defective tissue or degenerated cells to treat different diseases. The adipose tissue and amniotic membrane have awakened great interest in regenerative medicine and arises as a promising source of mesenchymal stem cells. Both types, adipose and amniotic derived mesenchymal stem cells (AMSCs) are multipotent cells with an enhanced ability to differentiate into multiple lineages.. We aimed to evaluate the effect of basal supplementation of exosomes in cell cultures with canine amniotic mesenchymal stem cells (MSCs). Mesenchymal stem cells derived from canine amniotic and adipose tissue were isolated and cultured performing cell passages until 80-90% confluence was reached. The growth curve was determined and peak cell growth was observed in the second passage. The cells were then characterized and differentiated into adipogenic, chondrogenic and osteogenic lineages. Extracel-lular vesicles from amnion were isolated using an ultracentrifugation protocol and characterized by nanosight analysis. To evaluate their ability to improve cellular viability in naturally inefficient passages, exosomes were co-cultures to the MSC cells. The results showed a 15-20% increase in the expansion rate of cultures supplemented with vesicles extracted in the first and second passages when compared to the control group. Statistical analysis using the Dunnett test (p <= 0.05) corroborated this result, showing a positive correlation between supplementation and expansion rate. These results indicate not only the importance of exosomes in the cell communication process but also the feasibility of the culture sup-plementation protocol for therapeutic purposes. The potential of the AMSCs for reproductive biotech-nology is undoubted, however, their application to repair reproductive disorders and the involved mechanisms remain elusive. The strategies to enable the Adipose Stem Cells and AMSCs application in reproductive biotechnology and optimize their use for tissue regeneration open new venues using exosomes interactions. (c) 2022 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 17/21266-0 - Células progenitoras amnióticas e seu potencial terapêutico
• Beneficiário: Carlos Eduardo Ambrósio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/04928-7 - 19th International Congress on Animal Reproduction ICAR
• Beneficiário: Carlos Eduardo Ambrósio
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 19/23840-0 - Potencialização da produção ovariana de fêmeas bovinas utilizando vesículas extracelulares derivadas de células-tronco mesenquimais: compreensão dos efeitos terapêuticos e análise de custo-benefício
• Beneficiário: Juliano Coelho da Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular