Oxidized hyaluronic acid/adipic acid dihydrazide hydrogel as cell microcarriers for tissue regeneration applications

Autor(es)

Franca, Carla Giometti ; Leme, Krissia Caroline ; Malheiros Luzo, Angela Cristina ; Hernandez-Montelongo, Jacobo ; Andrade Santana, Maria Helena

• Tipo de documento: Artigo Científico
• Fonte: E-POLYMERS; v. 22, n. 1, p. 10-pg., 2022-12-07.

Resumo

Hyaluronic acid (HA) is a biopolymer present in various human tissues, whose degradation causes tissue damage and diseases. The oxidized hyaluronic acid/adipic acid dihydrazide (oxi-HA/ADH) hydrogels have attracted attention due to their advantages such as thermosensitivity, injectability, in situ gelation, and sterilization. However, studies are still scarce in the literature as microcarriers. In that sense, this work is a study of oxi-HA/ADH microparticles of 215.6 & PLUSMN; 2.7 mu m obtained by high-speed shearing (18,000 rpm at pH 7) as cell microcarriers. Results showed that BALB/c 3T3 fibroblasts and adipose mesenchymal stem cells (h-AdMSC) cultured on the oxi-HA/ADH microcarriers presented a higher growth of both cells in comparison with the hydrogel. Moreover, the extrusion force of oxi-HA/ADH microparticles was reduced by 35% and 55% with the addition of 25% and 75% HA fluid, respectively, thus improving its injectability. These results showed that oxi-HA/ADH microcarriers can be a potential injectable biopolymer for tissue regeneration applications. (AU)

• Processo FAPESP: 16/10132-0 - Plasma-rico em plaquetas associado ao ácido hialurônico para aplicações em ortopedia regenerativa: do desenvolvimento tecnológico das preparações às caracterizações físico-químicas e biológicas in vitro
• Beneficiário: Maria Helena Andrade Santana
• Modalidade de apoio: Auxílio à Pesquisa - Regular