| Texto completo | |
| Autor(es): |
Sellera, Fabio P.
;
Lopes, Ralf
;
Monte, Daniel F. M.
;
Cardoso, Brenda
;
Esposito, Fernanda
;
dos Anjos, Carolina
;
da Silva, Luciano C. B. A.
;
Lincopan, Nilton
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 22, p. 3-pg., 2020-09-01. |
| Resumo | |
Background: Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae have been increasingly reported worldwide as a frequent cause of human and animal infections. K. pneumoniae belonging to the K24 capsular serotype and sequence type (ST) ST15 has been considered a global successful clone responsible for the spread of the bla(CTX-M-15) gene. Objective: To report the draft genome sequence of a multidrug-resistant CTX-M-15-positive K. pneumoniae K24-ST15 strain (L3KP1), which was isolated from a dog with chronic otitis. Methods: Genomic DNA was extracted and sequenced using Illumina NextSeq platform. De novo assembly was performed by SPAdes and in silico prediction accomplished by curated bioinformatics tools. Results: The genome size was calculated at 5 642 348 bp, with a GC content of 57.11%, and comprising 5601 total genes, 52 tRNAs, 8 rRNAs, 9 ncRNAs and 105 pseudogenes. The K. pneumoniae L3KP1 strain belonged to ST15 and carried the yersiniabactin biosynthetic gene cluster [ybt 10 (YbST28) in the integrative conjugative element ICEKp4], and the KL24 locus encoding capsular serotype K24. Besides the bla(CTX-M-15) ESBL gene, other clinically important resistance genes to beta-lactams, aminoglycosides, fosfomycin, macrolides, phenicol, quinolones, sulfonamides, tetracyclines and trimethoprim were detected. Additionally, heavy metals and disinfectant resistance genes were also identified. Conclusion: This draft genome might be useful for comparative genomic analyses of the international clone of K. pneumoniae K24-ST15-CTX-M-15. In addition, information presented in this study also shed light on the urgent need to monitor ESBL-producing K. pneumoniae in veterinary hospitals. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. (AU) | |
| Processo FAPESP: | 16/08593-9 - Pan-resistoma de Klebsiella pneumoniae e Escherichia coli produtoras de beta-lactamases (KPC-2, CTX-M-8, CTX-M-15) endêmicas no Brasil |
| Beneficiário: | Nilton Erbet Lincopan Huenuman |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/03044-7 - Diversidade genética, genes de virulência e resistência antimicrobiana de isolados de Salmonella spp. provenientes de abatedouros de frango |
| Beneficiário: | Daniel Farias Marinho Do Monte |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |