In vitro and in vivo persistence of IncN plasmids carrying qnr genes in uropathogenic Escherichia coli isolates

Autor(es)

Segura, Wilson Dias ; Ramos, Haissa Pereira ; de Faria Blanc Amorim, Renata Ester ; da Silva Ribeiro, Aghata Cardoso ; Pereira, Edimar Cristiano ; Cayo, Rodrigo ; Gales, Ana Cristina ; Piantino Ferreira, Antonio J. ; da Rocha Minarini, Luciene Andrade

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 22, p. 5-pg., 2020-09-01.

Resumo

Objectives: This study aimed to evaluate the persistence of the plasmid-mediated quinolone resistance (PMQR) among uropathogenic Escherichia coli strains grown under or without exposure to subinhibitory concentrations of ciprofloxacin. Based on that, we evaluated the possible spontaneous loss or maintenance of PMQR and the possible appearance of compensatory mutations in gyrA and parC genes. Methods: Three uropathogenic E. coli strains harbouring chromosomal mutations in the gyrA and/or parC genes coupled with qnrS1 or qnrB2 determinants carried by distinct plasmid sizes and incompatibility N groups (IncN/ST1, IncN/STS) were evaluated using in vitro and in vivo assays. Results: PMQRs remained stable in all strains throughout the generations evaluated, independently of exposure to ciprofloxacin in both in vivo and in vitro assays. Analysis of gyrA and parC genes after in vivo and in vitro assays revealed that no changes occurred in quinolone-resistance determining regions (QRDR). Conclusion: We demonstrated that IncN plasmids were persistent over 14 days in E. coli clinical strains independently of exposure to ciprofloxacin, as well as previous mutations in QRDR. (C) 2020 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. (AU)

• Processo FAPESP: 12/18741-4 - Caracterização molecular de plasmídeos de amostras clínicas brasileiras carreadoras do gene qnr
• Beneficiário: Luciene Andrade da Rocha Minarini
• Modalidade de apoio: Auxílio à Pesquisa - Regular