Proteomic dataset of Listeria monocytogenes exposed to sublethal concentrations of free and nanoencapsulated nisin

Autor(es)

Pinilla, Cristian Mauricio Barreto ; Stincone, Paolo ; Brandelli, Adriano

• Tipo de documento: Artigo Científico
• Fonte: DATA IN BRIEF; v. 43, p. 10-pg., 2022-06-04.

Resumo

The cellular proteins of L. monocytogenes exposed to free and liposome-encapsulated nisin at sublethal concentration were hydrolyzed by trypsin and examined by tandem mass spectrometry (MS/MS) to obtain proteomic data. In the present study, we use the STRING v11.05 database analyze the interactions among the 78 upregulated proteins from L. monocytogenes obtained after treatment with sublethal concentrations of free and nanoliposome-encapsulated nisin. As result, from the upregulated proteins by free nisin was determined a network with 140 edges with two relevant nodes, containing ribosomal proteins and transmembrane transport proteins (SecD and ABC transport system). These two sets of proteins present biological connection as a group, with strong interactions and are related to detoxification and other Listeria response mechanisms. In addition, a high amount of membrane proteins was identified in the free nisin treatment. On the other hand, in the interaction analysis of upregulated proteins by liposome-loaded nisin, was found 156 edges with a single protein network, the same observed in free nisin, related to ribosomal proteins. Therefore, according with this analysis, the encapsulation of nisin into liposomes cause upregulation of ribosomal and decrease of L. monocytogenes response proteins as compared with free nisin. (C) 2022 The Author(s). Published by Elsevier Inc. (AU)

• Processo FAPESP: 21/04987-0 - Produção de biomassa de bactérias ácido láticas autóctones em meios a base de diferentes tipos de soro de leite e otimização de processos de secagem
• Beneficiário: Cristian Mauricio Barreto Pinilla
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado