Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A

Autor(es)

Dellaqua, Thaisy Tino ; Franchi, Fernanda Fagali ; dos Santos, Priscila Helena ; Giroto, Alan Brunholi ; Nunes, Sarah Gomes ; de Lima, Victor Augusto Vieira ; Guilherme, Vitoria Bertogna ; Fontes, Patricia Kubo ; Sudano, Mateus Jose ; Castilho, Anthony Cesar de Souza

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY RESEARCH COMMUNICATIONS; v. N/A, p. 10-pg., 2023-01-18.

Resumo

Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using in vitro-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (P > 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating PPARGC1A and AKR1B1, which are related to lipid metabolism; CASP9, a pro-apoptotic gene; and IFN-tau, a marker of embryo quality (P < 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (P < 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A's approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts. (AU)

• Processo FAPESP: 17/19679-4 - Efeito da adição da proteína sérica a associada à prenhez (PAPP-A) sobre o perfil e metabolismo lipídico de oócitos bovinos maturados in vitro
• Beneficiário: Thaisy Tino Dellaqua
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/06454-7 - Impacto da adição da proteína sérica a associada à prenhez (PAPP-A) durante a maturação oocitária in vitro sobre o perfil e metabolismo lipídico de embriões bovinos
• Beneficiário: Victor Augusto Vieira de Lima
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica