| Texto completo | |
| Autor(es): |
de Melo, Bruna A. G.
;
Jodat, Yasamin A.
;
Mehrotra, Shreya
;
Calabrese, Michelle A.
;
Kamperman, Tom
;
Mandal, Biman B.
;
Santana, Maria H. A.
;
Alsberg, Eben
;
Leijten, Jeroen
;
Shin, Su Ryon
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ADVANCED FUNCTIONAL MATERIALS; v. 29, n. 51, p. 13-pg., 2019-10-21. |
| Resumo | |
Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, while locomotive forces demand tissues with strong mechanical properties, chondrogenesis requires a soft microenvironment. To address this challenge, 3D cartilage-like tissue is fabricated using two biomaterials with different mechanical properties: a hard biomaterial to reflect the macromechanical properties of native cartilage, and a soft biomaterial to create a chondrogenic microenvironment. To this end, a bath composed of an interpenetrating polymer network (IPN) of polyethylene glycol (PEG) and alginate hydrogel (MPa order compressive modulus) is developed as an extracellular matrix (ECM) with self-healing properties. Within this bath supplemented with thrombin, human mesenchymal stem cell (hMSC) spheroids embedded in fibrinogen are 3D bioprinted, creating a soft microenvironment composed of fibrin (kPa order compressive modulus) that simulate cartilage's pericellular matrix and allow a fast diffusion of nutrients. The bioprinted hMSC spheroids present high viability and chondrogenic-like behavior without adversely affecting the macromechanical properties of the tissue. Therefore, the ability to locally bioprint a soft and cell stimulating biomaterial inside of a mechanically robust hydrogel is demonstrated, thereby uncoupling the micro- and macromechanical properties of the 3D printed tissues such as cartilage. (AU) | |
| Processo FAPESP: | 17/02913-4 - Microfluídica para cultura 3D de h-AdMSCs em ácido hialurônico livre ou estruturado em esponjas: quimiotaxia e quimiocinese em resposta a gradientes de fatores de crescimento de L-PRP |
| Beneficiário: | Bruna Alice Gomes de Melo |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |