Gene expression changes in Epinephelus marginatus (Teleostei, Serranidae) liver reveals candidate molecular biomarker of iron ore contamination

Autor(es)

Gomes, Aline Silva ; Passos, Larissa Souza ; Rocha Aride, Paulo Henrique ; Chiste, Barbara ; Gomes, Levy Carvalho ; Boldrini-Franca, Johara

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 303, p. 9-pg., 2022-05-26.

Resumo

Wastes from iron ore mining activities are potentially damaging to adjacent aquatic ecosystems. We aimed to determine biomarkers of environmental exposure to this xenobiotic in the dusky grouper Epinephelus marginatus by differential gene expression analysis. For this, fish were exposed to iron ore (15.2 mg/L) and gene expression in liver was assessed by RNA-Seq and compared to the control group. A total of 124 differentially expressed genes were identified, from which 52 were upregulated and 72 were downregulated in response to iron ore. From these, ferritin (medium subunit), cytochrome b reductase and epoxide hydrolase genes were selected for validation by RT-qPCR that confirmed the upregulation of epoxide hydrolase in fish exposed to iron ore. (AU)

• Processo FAPESP: 20/00109-6 - Avaliação da toxicidade de cianopeptídeos em peixes para consumo humano provenientes de pisciculturas em tanques-rede
• Beneficiário: Larissa Souza Passos
• Modalidade de apoio: Bolsas no Brasil - Mestrado