Photobiomodulation therapy's effects on cardiac fibrosis activation after experimental myocardial infarction

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Feliciano, Regiane dos S. ; Manchini, Martha T. ; Atum, Allan L. B. ; Silva, Gabriel Almeida ; Antonio, Ednei L. ; Serra, Andrey J. ; Tucci, Paulo J. F. ; de Mello, Ramon Andrade ; Chavantes, Maria C. ; Baltatu, Ovidiu C. ; Silva Junior, Jose A.

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Surgery and Medicine; v. 54, n. 6, p. 12-pg., 2022-04-02.

Resumo

Introduction Ischemic heart disease is the leading cause of death worldwide, and interventions to reduce myocardial infarction (MI) complications are widely researched. Photobiomodulation therapy (PBMT) has altered multiple biological processes in tissues and organs, including the heart. Objectives This study aimed to assess the temporal effects of PBMT on cardiac fibrosis activation after MI in rats. In this proof-of-concept study, we monitored the change in expression patterns over time of genes and microRNAs (miRNAs) involved in the formation of cardiac fibrosis post-MI submitted to PBMT. Materials and Methods Experimental MI was induced, and PBMT was applied shortly after coronary artery ligation (laser light of wavelength 660 nm, 15 mW of power, energy density 22.5 J/cm(2), 60 seconds of application, irradiated area 0.785 cm(2), fluence 1.1 J/cm(2)). Ventricular septal samples were collected at 30 minutes, 3, 6, 24 hours, and 3 days post-MI to determine temporal PBMT's effects on messenger RNA (mRNA) expression associated with cardiac fibrosis activation and miRNAs expression. Results PBMT, when applied after ischemia, reversed the changes in mRNA expression of myocardial extracellular matrix genes induced by MI. Surprisingly, PBMT modified cardiac miRNAs expression related to fibrosis replacement in the myocardium. Expression correlations between myocardial mRNAs were assessed. The correlation coefficient between miRNAs and target mRNAs was also determined. A positive correlation was detected among miR-21 and transforming growth factor beta-1 mRNA. The miR-29a expression negatively correlated to Col1a1, Col3a1, and MMP-2 mRNA expressions. In addition, we observed that miR-133 and Col1a1 mRNA were negatively correlated. Conclusion The results suggest that PBMT, through the modulation of gene transcription and miRNA expressions, can interfere in cardiac fibrosis activation after MI, mainly reversing the signaling pathway of profibrotic genes. (AU)

• Processo FAPESP: 12/15808-0 - Caracterização da expressão gênica de vias de transdução do sinal no miocárdio remoto ao infarto induzido por ablação ventricular esquerda e oclusão da artéria coronária em ratos
• Beneficiário: Eduardo Tadeu Santana
• Modalidade de apoio: Bolsas no Brasil - Doutorado