Measuring concentrations of a dye in the hemolymph of a marine amphipod: Development of a protocol for exposure assessment

Autor(es)

dos Santos, Amanda ; Vannuci-Silva, Monizze ; Vendemiatti, Josiane A. de Souza ; Artal, Mariana Coletty ; da Silva, Bianca Ferreira ; Boldrin Zanoni, Maria Valnice ; Umbuzeiro, Gisela de Aragao

• Tipo de documento: Artigo Científico
• Fonte: Marine Pollution Bulletin; v. 175, p. 5-pg., 2022-02-04.

Resumo

The increasing pollution of aquatic environments due to old and emerging contaminants requires the develop-ment of integrative methods for exposure assessment. Internal concentrations are a reliable way to estimate total exposure of contaminants originated from different routes (water, sediment, and food). We developed a protocol to evaluate the concentration of a dye, C.I. Disperse Red 1, in the hemolymph of Parhyale hawaiensis, a marine amphipod. LOD and LOQ were satisfactory to detect the dye in all hemolymph samples. The concentration detected in the hemolymph varied related to exposure time and dye concentration (0.003 to 0.086 mu g mL(-1)). Polynomial regression model was the best fit. The protocol was reliable to detect and quantify dye exposure in marine amphipods and can be considered for future assessments of estuarine and marine regions under the in-fluence of dye processing plants. The method possibly can be easily adapted to other amphipods and other azo dyes. (AU)

• Processo FAPESP: 13/26301-7 - Desenvolvimento e aplicação de método para determinação de prata na hemolinfa de anfípodas expostos a nanomateriais
• Beneficiário: Monizze Vannuci e Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/08829-7 - Desenvolvimento de biomarcadores de ecotoxicidade no anfípoda marinho Parhyale hawaiensis e avaliação da toxicidade de nanomateriais
• Beneficiário: Mariana Coletty Artal
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/10449-7 - Avaliação da ocorrência, toxicidade/genotoxicidade e processos para degradação de corantes em efluentes e águas superficiais
• Beneficiário: Maria Valnice Boldrin
• Modalidade de apoio: Auxílio à Pesquisa - Temático