| Texto completo | |
| Autor(es): |
Sartori, Marina R.
;
Navarro, Claudia D. C.
;
Castilho, Roger F.
;
Vercesi, Anibal E.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 260, p. 8-pg., 2022-03-12. |
| Resumo | |
The occurrence of hepatic lipidosis is commonly reported in different reptilian species, especially in animals under captivity. Liver accumulation of fat is associated with disorders, better described in mammals as non-alcoholic fatty liver diseases (NAFLD), ranging from simple steatosis, to non-alcoholic steatohepatitis (NASH), and to more severe lesions of cirrhosis and hepatocellular carcinoma. Mitochondria play a central role in NAFLD pathogenesis, therefore in this study we characterized livers of ad libitum fed captive red-footed tortoise Chelo-noidis carbonaria through histological and mitochondrial function evaluations of juvenile and adult individuals. Livers from adult tortoises exhibited higher levels of lipids, melanomacrophages centers and melanin than ju-veniles. The observed high score levels of histopathological alterations in adult tortoises, such as microvesicular steatosis, inflammation and fibrosis, indicated the progression to a NASH condition. Mitochondrial oxygen consumption at different respiratory states and with different substrates was 30 to 58% lower in adult when compared to juvenile tortoises. Despite citrate synthase activity was also lower in adults, cardiolipin content was similar to juveniles, indicating that mitochondrial mass was unaffected by age. Mitochondrial Ca2+ retention capacity was reduced by 70% in adult tortoises. Overall, we found that aggravation of NAFLD in ad libitum fed captive tortoises is associated with compromised mitochondrial function, indicating a critical role of the organelle in liver disease progression in reptiles. (AU) | |
| Processo FAPESP: | 17/05487-6 - Funções mitocondriais e marcadores moleculares associados ao envelhecimento em uma espécie longeva, a tartaruga terrestre Chelonoidis carbonaria (Jabuti piranga) |
| Beneficiário: | Marina Rincon Sartori |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/17728-8 - Função e disfunção mitocondrial: implicações para o envelhecimento e doenças associadas |
| Beneficiário: | Aníbal Eugênio Vercesi |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/20855-7 - Papel da NAD(P)-Transidrogenase mitocondrial no desenvolvimento da Doença Hepática Gordurosa e no envelhecimento |
| Beneficiário: | Claudia Daniele Carvalho Navarro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |