Inhibition of sphingolipid de novo synthesis counteracts muscular dystrophy

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Laurila, Pirkka-Pekka ; Luan, Peiling ; Wohlwend, Martin ; Zanou, Nadege ; Crisol, Barbara ; de Lima, Tanes Imamura ; Goeminne, Ludger J. E. ; Gallart-Ayala, Hector ; Shong, Minho ; Ivanisevic, Julijana ; Place, Nicolas ; Auwerx, Johan

• Tipo de documento: Artigo Científico
• Fonte: SCIENCE ADVANCES; v. 8, n. 4, p. 14-pg., 2022-01-28.

Resumo

Duchenne muscular dystrophy (DMD), the most common muscular dystrophy, is a severe muscle disorder, causing muscle weakness, loss of independence, and premature death. Here, we establish the link between sphingolipids and muscular dystrophy. Transcripts of sphingolipid de novo biosynthesis pathway are up-regulated in skeletal muscle of patients with DMD and other muscular dystrophies, which is accompanied by accumulation of metabolites of the sphingolipid pathway in muscle and plasma. Pharmacological inhibition of sphingolipid synthesis by myriocin in the mdx mouse model of DMD ameliorated the loss in muscle function while reducing inflammation, improving Ca2+ homeostasis, preventing fibrosis of the skeletal muscle, heart, and diaphragm, and restoring the balance between M1 and M2 macrophages. Myriocin alleviated the DMD phenotype more than glucocorticoids. Our study identifies inhibition of sphingolipid synthesis, targeting multiple pathogenetic pathways simultaneously, as a strong candidate for treatment of muscular dystrophies. (AU)

• Processo FAPESP: 19/22512-0 - Efeitos da restrição calórica e do exercício físico sobre o mecanismo de agregação proteica no músculo esquelético
• Beneficiário: Barbara Moreira Crisol
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado