Arthrospira (Spirulina) platensis feeding reduces the early stage of chemically induced rat colon carcinogenesis

Autor(es)

Amadeu, Simone Oliveira ; Sarmiento-Machado, Luis Manuel ; Bartolomeu, Ariane Rocha ; Garcia Chaves, Maria Angel ; Romualdo, Guilherme Ribeiro ; de Moura, Nelci Antunes ; Barbisan, Luis Fernando

• Tipo de documento: Artigo Científico
• Fonte: BRITISH JOURNAL OF NUTRITION; v. N/A, p. 11-pg., 2022-05-04.

Resumo

Colorectal cancer is the third most diagnosed cancer worldwide and linked to dietary/lifestyle factors. Arthrospira (Spirulina) platensis (AP) contains bioactive compounds with beneficial effects in vivo/in vitro. We evaluated the effects of AP feeding against 1,2-dimethylhydrazine (DMH)-induced colon carcinogenesis. Male Sprague Dawley rats were given subcutaneous injections of DMH (4 x 40 mg/kg body weight) (G1-G3) or vehicle (G4-G5) twice a week (weeks 3-4). During weeks 1-4, animals were fed a diet containing 1 % (G2) or 2 % (G3-G4) AP powder (w/w). After this period, all groups received a balanced diet until week 12. Some animals were euthanised after the last DMH injection (week 4) for histological, immunohistochemical (Ki-67, gamma-H2AX and caspase-3) and molecular analyses (real time-PCR for 91 genes), while other animals were euthanised at week 12 for preneoplastic aberrant crypt foci (ACF) analysis. Both AP treatments (G2-G3) significantly decreased the DMH-induced increase in gamma-H2AX (DNA damage) and caspase 3 (DNA damage-induced cell death) in colonic crypts at week 4. In addition, Cyp2e1 (Drug metabolism), Notch1, Notch2 and Jag1 genes (Notch pathway) and Atm, Wee1, Chek2, Mgmt, Ogg1 and Xrcc6 genes (DNA repair) were also down-regulated by 2 % AP feeding (G3) at week 4. A significant reduction in ACF development was observed in both AP-treated groups (G2-G3) at week 12. In conclusion, findings indicate that AP feeding reduced acute colonic damage after DMH, resulting in fewer preneoplastic lesions. Our study provided mechanistic insights on dietary AP-preventive effects against early colon carcinogenesis. (AU)

• Processo FAPESP: 17/26217-7 - Ação de compostos bioativos do café em células tumorais e na modulação da expressão de miRNAs durante a carcinogênese de cólon
• Beneficiário: Ariane Rocha Bartolomeu
• Modalidade de apoio: Bolsas no Brasil - Doutorado