Recovery of cellulose and lignin from Eucalyptus by-product and assessment of cellulose enzymatic hydrolysis

Autor(es)

Cedeno, Fernando Roberto Paz ; de Siqueira, Breno Belon ; Chavez, Eddyn Gabriel Solorzano ; Roldan, Ismael Ulises Miranda ; Ropelato, Leonardo Moreira ; Galan, Julian Paul Martinez ; Masarin, Fernando

• Tipo de documento: Artigo Científico
• Fonte: RENEWABLE ENERGY; v. 193, p. 14-pg., 2022-05-21.

Resumo

In this study, a Eucalyptus by-product was fractionated into cellulose and lignin by pretreatment with NaClO2 to obtain alpha-cellulose, and hydrothermal pretreatment followed by sulfite-NaOH to obtain cellulose and lignin. The recovered celluloses were chemically and structurally characterized and enzymatically hydrolyzed using commercial enzyme preparations. The alpha-cellulose and cellulose treated with sulfite-NaOH had cellulose contents of 70.1% and 61.1%, respectively. alpha-cellulose hydrolyzed with Celluclast showed potential for cello-oligosaccharides formation, resulting in a maximum conversion of 30%, while Cellic CTec 2 resulted in 100% conversion to glucose, whereas cellulose treated with sulfite-NaOH showed potential for conversion to glucose with Cellulcast (44%) and Cellic CTec 2 (78%). The liquor from the sulfite-NaOH process resulted in a 20.8% yield of sulfonated lignin recovery. The use of different pretreatment techniques for the Eucalyptus by-product can be alternatives for the generation of products with high added value and commercial potential, such as cello-oligosaccharides, glucose, and sulfonated lignin.(c) 2022 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 18/06241-3 - Avaliação da produção de oligossacarídeos a partir de um subproduto de eucalyptus
• Beneficiário: Fernando Masarin
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/17690-6 - Imobilização de endoglucanases e xilanases de preparado enzimático comercial em óxido de grafeno magnetizado
• Beneficiário: Leonardo Moreira Ropelato
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/14254-8 - Avaliação da produção de glucooligossacarídeos por hidrólise enzimática de celulose de resíduo oriundo do processo de extração de hemicelulose de um subproduto de Eucalyptus
• Beneficiário: Breno Belon de Siqueira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica