Melanoma spheroid-containing artificial dermis as an alternative approach to in vivo models

Autor(es)

Monico, Danielli Azevedo ; Calori, Italo Rodrigo ; Souza, Carla ; Espreafico, Enilza Maria ; Bi, Hong ; Tedesco, Antonio Claudio

• Tipo de documento: Artigo Científico
• Fonte: Experimental Cell Research; v. 417, n. 1, p. 11-pg., 2022-05-20.

Resumo

Melanoma spheroid-loaded 3D skin models allow for the study of crucial tumor characteristics and factors at a superior level because the neoplastic cells are integrated into essential human skin components, permitting tumor-skin model communication. Herein, we designed a melanoma-containing artificial dermis by inserting multicellular tumor spheroids from the metastatic phase of WM 1617 melanoma cells into an artificial dermis. We cultured multicellular melanoma spheroids by hanging drop method (250 cells per drop) with a size of 420 mu m in diameter after incubation for 14 days. These spheroids were integrated into the dermal equivalents that had been previously preparedwith a type-I collagen matrix and healthy fibroblasts. The melanoma spheroid cells invaded and proliferated in the artificial dermis. Spheroids treated with a 1.0 mu mol/L aluminum chloride phthalocyanine nanoemulsion in the absence of light showed high cell viability. In contrast, under irradiation with visible red light (660 nm) at 25 J/cm(2), melanoma cells were killed and the healthy tissue was preserved, indicating that photodynamic therapy is effective in such a model. Therefore, the 3D skin melanoma model has potential to promote research in full-thickness skin model targeting optimized preclinical assays. (AU)

• Processo FAPESP: 20/04507-6 - "hidrogéis confinados em matriz impressa 3d para a entrega controlada e sítio-específica de ingenol-3-dodecanoato e fluorocromo visando fotodiagnóstico e terapia de tumores cerebrais"
• Beneficiário: Italo Rodrigo Calori
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/01272-5 - Modelos in vitro de pele reconstituída: desenvolvimento, avaliação dos danos e da eficácia fotoprotetora nas regiões UVA e visível do espectro solar
• Beneficiário: Carla Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado