Effect of MTTP-493G/T, I128T, Q95H and Q244E polymorphisms on hepatic steatosis in patients with chronic hepatitis

Autor(es)

Prata, Thamiris Vaz Gago ; Manchiero, Caroline ; Dantas, Bianca Peixoto ; Nunes, Arielle Karen da Silva ; Tengan, Fatima Mitiko ; Magri, Mariana Cavalheiro

• Tipo de documento: Artigo Científico
• Fonte: Clinics; v. 77, p. 8-pg., 2022-08-23.

Resumo

Background: Chronic hepatitis C is characterized by a progressive deterioration of liver function and is involved in metabolic complications, such as hepatic steatosis.Objective: The aim of this study was to investigate the role of host and viral characteristics associated with-493G/ T (rs1800591), I128T (rs3816873), Q95H (rs61733139), and Q244E (rs17599091) Single Nucleotide Polymor-phisms (SNPs) in the Microsomal Triglyceride Transfer Protein (MTTP) gene on hepatic steatosis in chronic hepatitis C.Methods: SNPs were genotyped by PCR-RFLP and analyzed in combination with host and viral characteristics by multiple logistic regression in different genetic models of inheritance.Results: The authors analyzed 236 patients with chronic hepatitis C, and 53% had hepatic steatosis. The mutated allele frequencies were > 5%, and the genotypes were in Hardy-Weinberg equilibrium (p >= 0.05). It was observed that patients with HCV genotype 3 infection (OR = 2.74, 95% CI 1.24-6.06, p = 0.013), female sex (OR = 2.28, 95% CI 1.21-4.28, p = 0.011) and moderate-and high-intensity liver inflammatory activity (A2-A3) (OR = 3.61, 95% CI 1.86-7.01, p < 0.001) alone exhibited a higher risk of steatosis. The results of multiple logistic regression analysis for interaction showed that for the-493G/T SNP, when the GT/TT genotype (dominant model) and the GT genotype (codominant model) were each combined with HCV genotype 3 infection, an 11.51-fold (95% CI 2.08-63.59, p = 0.005) and a 15.69-fold (95% CI 2.46-99.85, p = 0.004) increased risk of steatosis, respectively, was observed. For the I128T SNP, when both the IT/TT genotype (dominant model) and the IT geno-type (codominant model) were combined with HCV genotype 3 infection, an 8.51-fold (95% CI 1.59-45.54, p = 0.012) and an 8.40 fold (95% CI 1.51-46.91, p = 0.015) increased risk of steatosis, respectively, was observed. Conclusion: The present study showed that the viral genotype combined with the-493G/T and I128T SNPs in the MTTP gene influences hepatic steatosis. (AU)

• Processo FAPESP: 16/19690-5 - Alterações genéticas no gene MTTP e sua relação com níveis de lipídeos plasmáticos e esteatose hepática em pacientes com hepatite C crônica
• Beneficiário: Mariana Cavalheiro Magri
• Modalidade de apoio: Auxílio à Pesquisa - Regular