Ginkgo biloba Extract Stimulates Adipogenesis in 3T3-L1 Preadipocytes

Autor(es)

Thomaz, Fernanda Malanconi ; Simao, Jussara de Jesus ; da Silva, Viviane Simoes ; Forcelini Machado, Meira Maria ; Oyama, Lila Missae ; Ribeiro, Eliane Beraldi ; Cardoso Alonso Vale, Maria Isabel ; Telles, Monica Marques

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICALS; v. 15, n. 10, p. 14-pg., 2022-10-01.

Resumo

Smaller adipocytes are related to the reversal of metabolic disorders, suggesting that molecules that can act in the adipogenesis pathway are of great interest. The objective of this study was to investigate the effect of Ginkgo biloba extract (GbE) in modulating the differentiation in preadipocytes. 3T3-L1 preadipocytes were differentiated for 7 days into adipocytes without (control group) and with GbE at 1.0 mg/mL. Lipid content and gene expression were analyzed on day 7 (D7) by Oil Red O staining and PCR Array Gene Expression. Western blotting analysis of the key adipogenesis markers was evaluated during the differentiation process at days 3 (D3), 5 (D5), and 7 (D7). GbE increased lipid content and raised the gene expression of the main adipogenesis markers. Key proteins of the differentiation process were modulated by GbE, since C/EBP beta levels were decreased, while C/EBP alpha levels were increased at D7. Regarding the mature adipocytes' markers, GbE enhanced the levels of both FABP4 at D5, and perilipin at D3 and D5. In summary, the present findings showed that GbE modulated the adipogenesis pathway suggesting that the treatment could accelerate the preadipocyte maturation, stimulating the expression of mature adipocyte proteins earlier than expected. (AU)

• Processo FAPESP: 14/18929-9 - Investigação de potenciais mecanismos de ação do extrato padronizado de Ginkgo biloba (EGB) envolvidos na melhora da obesidade e resistência à insulina induzidas por dieta hiperlipídica
• Beneficiário: Monica Marques Telles
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/13618-9 - Estudo da modulação da expressão de Ezh2 e de outros modificadores de H3K27 por fatores da transcrição NF-kB em células adiposas
• Beneficiário: Maria Isabel Cardoso Alonso-Vale
• Modalidade de apoio: Auxílio à Pesquisa - Regular