Local administration of curcumin-loaded nanoparticles enhances periodontal repair in vivo

Autor(es)

Perez-Pacheco, Cindy Grace ; Rodrigues Fernandes, Natalie Aparecida ; Camilli, Angelo Constantino ; Ferrarezi, Danilo Paschoal ; Silva, Amanda Favoreto ; Zunareli, Mayara Cristina ; Amantino, Camila Fernanda ; Primo, Fernando Lucas ; Guimaraes-Stabilli, Morgana Rodrigues ; Rossa Junior, Carlos

• Tipo de documento: Artigo Científico
• Fonte: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY; v. 396, n. 2, p. 11-pg., 2022-11-03.

Resumo

The aim was to assess the influence of local application of curcumin-loaded nanoparticles on an experimental model of periodontal repair. Periodontitis was induced by ligatures on both lower first molars of rats. After 15 days, ligatures were removed ("treatment") and animals were randomly allocated to three experimental groups (n = 8/group): (i) 0.05 mg/ml curcumin-loaded nanoparticles, (ii) empty nanoparticles (vehicle control), and (iii) sterile saline (negative control). Experimental treatments were administered locally on days 0, 3, 5, 7, 9, and 11 after ligature removal. Animals were euthanized at 7 and 14 days. Bone repair was assessed by microcomputer tomography (mu CT). Histological sections were stained with hematoxylin/eosin (H/E), Picrosirius Red, and Masson's trichrome. Expression of Runx-2 was studied by immunohistochemistry. Gene expression of Itgam, Arg1, and Inos was assessed by RT-qPCR. At 7 days, there was increased gene expression of Itgam and Arg1 and of the relative expression of Arg1/Inos in curcumin-treated animals, but no difference in any other outcomes. At 14 days, curcumin-loaded nanoparticles significantly increased bone repair and collagen content, as well as the number of osteocytes, percentage of extracellular matrix, and expression of Runx2. The results demonstrate that local administration of curcumin-loaded nanoparticles enhanced tissue repair in an experimental model of periodontal repair. Nanoparticle-encapsulated curcumin enhances early post-treatment repair of periodontal tissues. (AU)

• Processo FAPESP: 20/11916-0 - Efeito da aplicação tópica de curcumin veiculado por nanoparticulas no reparo de tecido conjuntivo periodontal não mineralizado: estudo in vitro
• Beneficiário: Mayara Cristina Zunareli
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica