| Texto completo | |
| Autor(es): Mostrar menos - |
Martins, Renan Moura
;
Carvalho, Tamara
;
Bittar, Cintia
;
Quevedo, Daniela Muller
;
Miceli, Rafael Nava
;
Nogueira, Mauricio Lacerda
;
Ferreira, Helena Lage
;
Costa, Paulo Inacio
;
Araujo, Joao Pessoa, Jr.
;
Spilki, Fernando Rosado
;
Rahal, Paula
;
Calmon, Marilia Freitas
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Viruses-Basel; v. 14, n. 11, p. 10-pg., 2022-11-01. |
| Resumo | |
Wastewater-based epidemiology (WBE) is a tool involving the analysis of wastewater for chemicals and pathogens at the community level. WBE has been shown to be an effective surveillance system for SARS-CoV-2, providing an early-warning-detection system for disease prevalence in the community via the detection of genetic materials in the wastewater. In numerous nation-states, studies have indicated the presence of SARS-CoV-2 in wastewater. Herein, we report the primary time-course monitoring of SARS-CoV-2 RNA in wastewater samples in Sao Jose do Rio Preto-SP/Brazil in order to explain the dynamics of the presence of SARS-CoV-2 RNA during one year of the SARS-CoV-2 pandemic and analyze possible relationships with other environmental parameters. We performed RNA quantification of SARS-CoV-2 by RT-qPCR using N1 and N2 targets. The proportion of positive samples for every target resulted in 100% and 96.6% for N1 and N2, respectively. A mean lag of -5 days is observed between the wastewater signal and the new SARS-CoV-2-positive cases reported. A correlation was found between the air and wastewater temperatures and therefore between the SARS-CoV-2 viral titers for N1 and N2 targets. We also observed a correlation between SARS-CoV-2 viral titers and media wastewater flow for the N1 target. In addition, we observed higher viral genome copies within the wastewater samples collected on non-rainy days for the N1 target. Thus, we propose that, based on our results, monitoring raw wastewater may be a broadly applicable strategy that might contribute to resolving the pressing problem of insufficient diagnostic testing; it may represent an inexpensive and early-warning method for future COVID-19 outbreaks, mainly in lower- and middle-income countries. (AU) | |
| Processo FAPESP: | 19/07784-3 - Estudo in vitro da ação de peptídeos sintéticos como antivirais contra CHIKV |
| Beneficiário: | Marilia de Freitas Calmon |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |