| Texto completo | |
| Autor(es): |
Esguicero, Andre L. H.
;
Bockmann, Flavio A.
Número total de Autores: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ICHTHYOLOGY AND HERPETOLOGY; v. 110, n. 3, p. 11-pg., 2022-10-01. |
| Resumo | |
The value of clearing and staining whole organisms to study vertebrate anatomy is unquestionable. These methods have been developed for over a century leading to protocols to prepare triple-stained specimens to differentiate between bones, cartilage, and nerves. Despite their potential to advance the field of comparative anatomy, nerve-staining methods have been used by a small number of vertebrate systematists in part because of the inconsistently successful preparations. Here, we report on several modifications to the current Sudan black B protocols and propose a new acidfree protocol to differentiate among bone, cartilage, and nerves in whole small vertebrates. This method may also be used to stain solely bone and cartilage by eliminating the nerve-staining steps. The technique herein described is successful for preparing juveniles and adults (including miniatures). (AU) | |
| Processo FAPESP: | 16/50375-9 - Composição taxonômica da ictiofauna do alto rio Paraguai e do alto rio Juruena e suas relações biogeográficas |
| Beneficiário: | Flávio Alicino Bockmann |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |