Caspofungin alone or combined with polymyxin B are effective against mixed biofilm of Aspergillus fumigatus and carbapenem-resistant Pseudomonas aeruginosa

Autor(es)

Fortes, Bruna Nakanishi ; Scheunemann, Gaby ; Melo, Analy Salles de Azevedo ; Ishida, Kelly

• Tipo de documento: Artigo Científico
• Fonte: Research in Microbiology; v. 174, n. 1-2, p. 9-pg., 2023-01-01.

Resumo

Aspergillus fumigatus and Pseudomonas aeruginosa biofilms are associated to the recalcitrant and persistent infections due to resistance to antimicrobials. Here, we evaluated the effect of antimicrobials on single and mixed biofilms of A. fumigatus and P. aeruginosa (carbapenem-resistant and susceptible strains) determining total biomass by crystal violet, cell viability by colony forming unit count, and microscopy. Polymyxin B (PMB) had the best action on P. aeruginosa biofilms inhibiting the biomass (2-4 mg/mL) and it was efficient reducing the viable bacterial cells. Amphotericin B (AMB) and caspofungin (CAS) were the best antifungal at inhibiting A. fumigatus biofilms and reducing fungal viability at con-centration >= 1 and >= 16 mg/mL, respectively. In addition, CAS was able to significantly reduce P. aeruginosa viability in mixed biofilms. CAS combined with PMB also significantly reduced the mixed biofilm biomass and fungal and bacterial viability mainly against carbapenem-resistant bacterium. The light and fluo-rescence microscopy showed alterations on hyphae morphology and confirmed the increase of fungal and bacterial death cells after combined therapy of mixed biofilms. Taken together, our work showed that CAS alone and its combination with PMB showed better potential in reducing mixed biofilm biomass and fungal and bacterial viability, even for the carbapenem-resistant P. aeruginosa strain. (c) 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

• Processo FAPESP: 17/26076-4 - Efeito dos antimicrobianos na infecção polimicrobiana de Aspergillus fumigatus e Pseudomonas aeruginosa
• Beneficiário: Bruna Nakanishi Fortes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/19374-9 - Equinocandinas no controle de infecções associadas a biofilmes polimicrobianos de Candida spp. e bactérias como Stapholococcus aureus e Pseudomonas aeruginosa
• Beneficiário: Kelly Ishida
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/11486-5 - Desenvolvimento de uma estrutura polimérica tridimensional para avaliar os efeitos de antifúngicos na interação com Aspergillus fumigatus
• Beneficiário: Bruna Nakanishi Fortes
• Modalidade de apoio: Bolsas no Brasil - Mestrado