Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum

Autor(es)

Castro, Karla R. ; Setti, Grazielle O. ; Oliveira, Tassia R. de ; Rodrigues-Jesus, Monica J. ; Botosso, Viviane F. ; Araujo, Ana P. Perini de ; Durigon, Edson L. ; Ferreira, Luis C. S. ; Faria, Ronaldo C.

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 256, p. 8-pg., 2023-02-02.

Resumo

Zika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. An infected person may be asymptomatic or present symptoms such as fever, arthralgia, and in pregnancy it may lead to neurological disorders in the fetus, such as microcephaly. Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ANS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. A linear response was obtained for a wide concentration range from 0.01 to 9.80 x 105 pg mL-1 (r2 = 0.982), with a limit of detection of 0.48 pg mL-1. The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV. (AU)

• Processo FAPESP: 18/04332-1 - Desenvolvimento de dispositivo imunossensor descartável à base de eletrodos serigrafados para detecção eletroquímica de anticorpos anti-Zika vírus visando o diagnóstico da doença
• Beneficiário: Karla Patrícia Ribeiro Castro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/20045-7 - Descoberta de antígenos e desenvolvimento de métodos de diagnóstico sorológico e estratégias vacinais contra o Vírus Zika (ZIKV)
• Beneficiário: Luis Carlos de Souza Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático