The Isoflavanoid (+)-PTC Regulates Cell-Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line

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de Farias, Kaio Moraes ; Rosa-Ribeiro, Rafaela ; Souza, Edmarcia E. ; Kobarg, Jorg ; Banwell, Martin G. ; Vieira Neto, Jose Brito ; Alves Sales, Sarah Leyenne ; Ribeiro Costa, Paulo Roberto ; dos Santos, Rafael Cavalcante ; Gaspar, Francisco Vilaca ; Barreto Junior, Amaro Gomes ; Ferreira Oliveira, Maria Conceicao ; de Moraes, Manoel Odorico ; Furtado, Cristiana Libardi M. ; Carvalho, Hernandes F. ; Pessoa, Claudia

• Tipo de documento: Artigo Científico
• Fonte: CHEMISTRY & BIODIVERSITY; v. 19, n. 5, p. 15-pg., 2022-04-29.

Resumo

Prostate cancer is the second most common malignancy in men and the development of effective therapeutic strategies remains challenging when more advanced, androgen-independent or insensitive forms are involved. Accordingly, we have evaluated, using flow cytometry, confocal microscopy and image analysis, the anti-proliferative effects of (+)-2,3,9-trimethoxypterocarpan [(+)-PTC, 1] on relevant human prostate cancer cells as well as its capacity to control mitosis within them. In particular, the studies reported herein reveal that (+)-PTC exerts anti-proliferative activity against the PC-3 cell lines by regulating cell-cycle progression with mitosis being arrested in the prophase or prometaphase. Furthermore, it emerges that treatment of the target cells with this compound results in the formation of monopolar spindles, disorganized centrosomes and extensively disrupted gamma-tubulin distributions while centriole replication remains unaffected. Such effects suggest (+)-PTC should be considered as a possible therapy for androgen-insensitive/independent prostate cancer. (AU)

• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático