The C-Terminal Domain of Staphylococcus aureus Zinc Transport Protein AdcA Binds Plasminogen and Factor H In Vitro

Autor(es)

Salazar, Natalia ; Yamamoto, Bruno Bernardi ; Lima de Souza, Matilde Costa ; da Silva, Ludmila Bezerra ; Mattos Areas, Ana Paula ; Barbosa, Angela Silva

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS; v. 11, n. 2, p. 15-pg., 2022-02-01.

Resumo

Bacterial acquisition of metals from a host is an essential attribute to facilitate survival and colonization within an infected organism. Staphylococcus aureus, a bacterial pathogen of medical importance, has evolved its strategies to acquire multiple metals, including iron, manganese, and zinc. Other important strategies for the colonization and infection of the host have been reported for staphylococci and include the expression of adhesins on the bacterial surface, as well as the acquisition of host plasminogen and complement regulatory proteins. Here we assess the ability of the zinc transport protein AdcA from Staphylococcus aureus, first characterized elsewhere as a zinc-binding protein of the ABC (ATP-binding cassette) transporters, to bind to host molecules. Like other staphylococcus ion-scavenging proteins, such as MntC, a manganese-binding protein, AdcA interacts with human plasminogen. Once activated, plasmin bound to AdcA cleaves fibrinogen and vitronectin. In addition, AdcA interacts with the human negative complement regulator factor H (FH). Plasminogen and FH have been shown to bind to distinct sites on the AdcA C-terminal portion. In conclusion, our in vitro data pave the way for future studies addressing the relevance of AdcA interactions with host molecules in vivo. (AU)

• Processo FAPESP: 16/05878-2 - Identificação de novos fatores de virulência de leptospira envolvidos nos processos de adesão, invasão e resistência ao soro
• Beneficiário: Angela Silva Barbosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/17534-6 - Treinamento em técnicas de Biologia Molecular, Microbiologia e Imunologia
• Beneficiário: Bruno Bernardi Yamamoto
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico