[10]-gingerol induces apoptosis and inhibits metastatic dissemination of triple negative breast cancer invivo

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Martin, Ana Carolina B. M. ; Fuzer, Angelina M. ; Becceneri, Amanda B. ; da Silva, James Almada ; Tomasin, Rebeka ; Denoyer, Delphine ; Kim, Soo-Hyun ; McIntyre, Katherine A. ; Pearson, Helen B. ; Yeo, Belinda ; Nagpal, Aadya ; Ling, Xiawei ; Selistre-de-Araujo, Heloisa S. ; Vieira, Paulo Cezar ; Cominetti, Marcia R. ; Pouliot, Normand

• Tipo de documento: Artigo Científico
• Fonte: ONCOTARGET; v. 8, n. 42, p. 12-pg., 2017-09-22.

Resumo

There is increasing interest in the use of non-toxic natural products for the treatment of various pathologies, including cancer. In particular, biologically active constituents of the ginger oleoresin (Zingiber officinale Roscoe) have been shown to mediate anti-tumour activity and to contribute to the anti-inflammatory, antioxidant, antimicrobial, and antiemetic properties of ginger. Here we report on the inhibitory properties of [10]-gingerol against metastatic triple negative breast cancer (TNBC) in vitro and in vivo. We show that [10]-gingerol concentration-dependently induces apoptotic death in mouse and human TNBC cell lines in vitro. In addition, [10]-gingerol is well tolerated in vivo, induces a marked increase in caspase-3 activation and inhibits orthotopic tumour growth in a syngeneic mouse model of spontaneous breast cancer metastasis. Importantly, using both spontaneous and experimental metastasis assays, we show for the first time that [10]-gingerol significantly inhibits metastasis to multiple organs including lung, bone and brain. Remarkably, inhibition of brain metastasis was observed even when treatment was initiated after surgical removal of the primary tumour. Taken together, these results indicate that [10]-gingerol may be a safe and useful complementary therapy for the treatment of metastatic breast cancer and warrant further investigation of its efficacy, either alone or in combination with standard systemic therapies, in pre-clinical models of metastatic breast cancer and in patients. (AU)

• Processo FAPESP: 13/00798-2 - A matriz extracelular no envelhecimento, no exercício e no microambiente tumoral
• Beneficiário: Heloisa Sobreiro Selistre de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/18908-6 - Mecanismos celulares e moleculares envolvidos na ação antiproliferativa do [10]-gingerol sobre células de tumor de mama
• Beneficiário: Angelina Maria Fuzer
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/24940-8 - Avaliação da eficácia de mudanças estruturais do [10]-gingerol em combinação com o quimioterápico doxorubicina para o tratamento de câncer de mama: estudos in vitro e in vivo
• Beneficiário: Márcia Regina Cominetti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/03771-5 - Identificação de genes supressores de metástase
• Beneficiário: Rebeka Tomasin
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado