High-resolution melting for detecting KRAS mutations in colorectal cancer

Autor(es)

Miranda, Raelson Rodrigues ; Silva, Tiago Donizetti ; Forones, Nora Manoukian

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL REPORTS; v. 11, n. 6, p. 5-pg., 2019-12-01.

Resumo

Colorectal cancer is a leading cause of cancer that may metastasize. KRAS gene sequence of exon 2 should be examined for identification of patients that can be treated with anti-EGFR. The aim of the present study was to evaluate the efficacy of high-resolution melting (HRM) to detect KRAS mutations in colorectal cancer (CRC) tumors. The exon 2 of KRAS was amplified from 47 adenocarcinoma CRC tissues. The tumors were subjected to high-resolution melt using quantitative PCR to identify wild-type and mutant subgroups. The results were compared to the mutations detected by next-generation sequences (NGS). The study included 47 patients, with a mean age of 62 years, of whom 24 patients were male. Most of the patients had stage II or stage III tumors. The mean melting temperatures for the wild-type and mutated group at exon 2 were 78.13C and 77.87C, respectively (P<0.001, 95% CI = 0.11-0.4). The sensitivity and specificity of high-resolution melting were 83.3 and 96.6%, respectively, with a high concordance between the NGS and HRM methods for detecting KRAS mutation in exon 2 (0.816; P=0.625). Thus, HRM could be used as an alternative method for detecting KRAS mutations in colorectal cancer tissue. (AU)

• Processo FAPESP: 13/19268-3 - Biomarcadores baseados em genômica e epigenômica que auxiliem na detecção precoce e monitoramento dos pacientes com câncer colorretal
• Beneficiário: Nora Manoukian Forones
• Modalidade de apoio: Auxílio à Pesquisa - Regular