| Texto completo | |
| Autor(es): |
Biazi, Luiz E.
;
Santos, Samantha C.
;
Neto, Antonio A. Kaupert
;
Sousa, Amanda S.
;
Soares, Lauren B.
;
Renzano, Eduardo
;
Velasco, Juliana
;
Rabelo, Sarita C.
;
Costa, Aline C.
;
Ienczak, Jaciane L.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BioEnergy Research; v. 15, n. 1, p. 14-pg., 2021-03-10. |
| Resumo | |
In this work, we propose an adaptation strategy for Scheffersomyces stipitis NRRL Y-7124 to increase its tolerance to inhibitors of hemicellulosic hydrolysates in the production of bioethanol. Sugarcane bagasse pretreated with 0.5% (v/v) of sulfuric acid at 140 degrees C for 15 min was used as feedstock. The microorganism was subjected to a cell recycle batch fermentation system with increased inhibitors pressure, characterized by five consecutive batches with 33% of hydrolysate, followed by five subsequent batches with 67% of hydrolysate. The remaining composition of the culture media for each sequential batch (necessary to complete 100% of carbohydrate content) was synthetic substrate. Finally, the strain was used in a fed-batch fermentation with 100% of hydrolysate, resulting in an adapted strain of S. stipitis at the end. In order to validate the adaptation strategy, the fermentative performances of the adapted and non-adapted strains were compared under different acetic acid concentrations using synthetic substrate. 0.7 g/L of acetic acid significantly affected the fermentative performance of the non-adapted strain, while for the adapted strain, this value was 3.7-fold higher (2.6 g/L), confirming the increased tolerance. Through proteomic analysis, genes related to the defense mechanisms of S. stipitis against oxidative stress due to the presence of inhibitory compounds were identified, which plays a key role on the comprehension of the cell adaptation metabolism. (AU) | |
| Processo FAPESP: | 14/01135-0 - Produção de bioetanol de segunda geração em biorreatores intensificados a partir de cepas de Scheffersomyces stipitis melhoradas por evolução induzida |
| Beneficiário: | Samantha Christine Santos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/14567-0 - Modelagem matemática do processo de produção de bioetanol de segunda geração com reciclo de células utilizando os microrganismos Scheffersomyces stipitis e Spathaspora passalidarum |
| Beneficiário: | Luiz Eduardo Biazi |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 15/20630-4 - Desenvolvimento integrado de biorrefinaria e planta de bioetanol de cana-de-açúcar com emissão zero de CO2: rotas para converter recursos renováveis em bioprodutos e bioeletricidade |
| Beneficiário: | Rubens Maciel Filho |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/04997-0 - Avaliação de leveduras nativas Scheffersomyces stipitis e Spathaspora passalidarum na produção de bioetanol de segunda geração a partir de hidrolisados de bagaço de cana-de-açúcar contendo xilose e glicose |
| Beneficiário: | Jaciane Lutz Ienczak |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |