Amperometric detection of indapamide on glassy carbon electrode

Autor(es)

Oliveira, Thawan G. ; Santos, Berlane G. ; Goncalves, Josue M. ; Angnes, Lucio

• Tipo de documento: Artigo Científico
• Fonte: Electroanalysis; v. 35, n. 9, p. 9-pg., 2023-05-11.

Resumo

This work describes the development of a fast, precise and reliable voltammetric method for the quantification of indapamide, an orally active diuretic sulfonamide used for hypertensive treatment. This compound acts inhibiting sodium reabsorption and increasing the elimination of water. This characteristic was responsible for its banishment by the International Olympic Committee since 1999. The study begins by finding an adequate potential range (-0.20 to 0.30 V) to avoid poisoning the working glassy carbon electrode (GCE) in phosphate buffer 0.10 mol L-1 (pH=12.0). Utilizing flow injection analysis, linear responses between 2.0 x 10(-6) mol L-1 to 2.5 x 10(-5) mol L-1 of indapamide (R-2=0.995), and detection limit (LOD) 3.0 x 10(-7) mol L-1 were obtained. This method was applied for the quantification of indapamide in tablets and in synthetic urine. The same flow system was used for the analysis of commercial drugs and the response obtained corresponded to 98 % of the concentration indicated on the drug label. These tablets were also analyzed by high performance liquid chromatography (HPLC), obtaining a recovery of 103 % and LOD 4.0 x 10(-7) mol L-1. The velocity of analysis using flow methods compares advantageously to the classical chromatographic methods. For synthetic urine, linear responses were obtained in samples spiked in the region from 5.0 x 10(-6) mol L-1 to 30 x 10(-6) mol L-1 (R-2=0.991) and LOD 3.0 x 10(-7) mol L-1. (AU)

• Processo FAPESP: 18/16896-7 - Desenvolvimento de sensores amperométricos e impedimétricos baseados em hidróxidos duplos de níquel e Vanádio (alfa-NixV1-x(OH)2) e seus nanocompósitos com grafite expansível/grafenos
• Beneficiário: Josué Martins Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/13137-5 - Formação e propriedades de hemiésteres do ácido carbônico em meio aquoso
• Beneficiário: Claudimir Lucio Do Lago
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático