Modern automated sample preparation for the determination of organic compounds: A review on robotic and on-flow systems

Autor(es)

Medina, Deyber Arley Vargas ; Maciel, Edvaldo Vasconcelos Soares ; Lancas, Fernando Mauro

• Tipo de documento: Artigo Científico
• Fonte: TRAC-TRENDS IN ANALYTICAL CHEMISTRY; v. 166, p. 21-pg., 2023-09-01.

Resumo

Sample preparation is crucial for reliable and confident analytical methods, ensuring selective enrichment, clean-up, and removal of matrix interferents. However, it often involves labor-intensive, error prone procedures. Automation of sample preparation improves throughput, precision, and accuracy while reducing health risks associated with hazardous chemicals or biological agents. Robotic systems and on-flow techniques are two common approaches for automating sample preparation in chromatography and mass spectrometry. Robotic systems are versatile automation platforms with mobile parts for performing chemical operations. Many commercial solutions are currently available, and open-source prototyping enables the affordable development and implementation of lab robots. On-flow techniques encompass a variety of strategies based on pumps and valves, with column-switching being particularly effective for the direct injection of raw samples and the online integration of extraction/preconcentration and separation stages. This review provides an update on the latest advances in sample preparation automation, introduces and explains the main automation strategies, highlights selected applications, and outlines their scope and limitations. Recent advancements in the use of robots and on-flow techniques in the automation of sample preparation for analyzing organic compounds via liquid chromatography and mass spectrometry are discussed, including applications, limitations, and future trends. Lastly, it presents trends and insights into future advancements.& COPY; 2023 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 19/22724-7 - Novas estratégias instrumentais no desenvolvimento de métodos analíticos automatizados e miniaturizados para a determinação de compostos orgânicos mediante cromatografia líquida acoplada a espectrometria de massas
• Beneficiário: Deyber Arley Vargas Medina
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/21984-0 - EMU concedido no processo 17/02147-0: sistema de nano-cromatografia líquida de alta eficiência hifenada a espectrometria de massas com ionização por elétrons e analisador quadrupolar (EI-Q (MS)) - nano-LC-EI-MS
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 17/21985-6 - EMU concedido no processo 17/02147-0: sistema de nano/micro-cromatografia líquida de alta eficiência hifenada a espectrometria de massas com ionização por electrospray e analisador sequencial quadrupolar (ESI-QqQ (MS/MS)) - nano/µ-LC-ESI-MS/MS
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários