| Texto completo | |
| Autor(es): Mostrar menos - |
Braga, Pollyana R. C.
;
Basso, Roberta M.
;
Martins, Lorrayne S. A.
;
Ribeiro, Marcio G.
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Amarante, Ariadne F.
;
Casas, Monique R. T.
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Nassar, Alessandra F. C.
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Pereira, Juliano G.
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Pantoja, Jose Carlos F.
;
Cerri, Fabricio M.
;
Oliveira-Filho, Jose P.
;
Borges, Alexandre S.
Número total de Autores: 12
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| Tipo de documento: | Artigo Científico |
| Fonte: | Pesquisa Veterinária Brasileira; v. 43, p. 12-pg., 2023-01-01. |
| Resumo | |
The present study investigated Salmonella spp. in the feces of 200 foals up to one year of age (100 with clinical signs of diarrhea and 100 without clinical signs of diarrhea). Bacteriological culture, serotyping, antimicrobial susceptibility, and real-time PCR (qPCR SYBR & REG; Green or a TaqMan & REG;) for detecting the invA gene (with and without a selective pre-enrichment step in tetrathionate broth) were performed. Bacterial culture revealed 15% (n=30) of positive animals (21 animals with diarrhea and nine without diarrhea). Among the 30 isolates, 13 different serovars were identified: S. Infantis, S. Minnesota, S. I.4,5,12:i:-; S. Anatum, S. Cerro, S. Oranienburg, S. Braenderup, S. Give, S. Newport, S. IIIb 61:c:z35, S. 109:-:1.5, S. I.4.12:d:-, S. I.6.8:-:-. Multidrug resistance was found in 43.33% (n=13) of the isolates, with one isolate obtained from animals without diarrhea and 12 isolates from animals with diarrhea. All qPCR techniques used in the study classified more samples as positive for Salmonella spp. than the bacterial culture of feces. In addition, all qPCR techniques detected more positive animals in the diarrhea group than in the diarrhea-free group. The results confirm the utility of the qPCR method without the pre-enrichment step in tetrathionate as a rapid test for Salmonella spp. in carrier animals. In animals with clinical signs of diarrhea, it can be combined with bacterial culture (antimicrobial susceptibility testing and serotyping). The isolation of Salmonella spp. in nine animals without diarrhea confirms the importance of asymptomatic carrier animals in the epidemiology of the disease. The multidrug resistance observed highlights the importance of rational antimicrobial use in horses and adopting biosecurity protocols that are efficacious in controlling the spread of infections between animals and zoonotic transmission in farms. (AU) | |
| Processo FAPESP: | 20/11957-8 - Estudo da diarreia em potros |
| Beneficiário: | Alexandre Secorun Borges |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |