Functional network of contextual and temporal memory has increased amygdala centrality and connectivity with the retrosplenial cortex, thalamus, and hippocampus

Autor(es)

Santos, Thays Brenner ; Kramer-Soares, Juliana Carlota ; Coelho, Cesar Augusto de Oliveira ; Menezes Oliveira, Maria Gabriela

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 13, n. 1, p. 23-pg., 2023-08-11.

Resumo

In fear conditioning with time intervals between the conditioned (CS) and unconditioned (US) stimuli, a neural representation of the CS must be maintained over time to be associated with the later US. Usually, temporal associations are studied by investigating individual brain regions. It remains unknown, however, the effect of the interval at the network level, uncovering functional connections cooperating for the CS transient memory and its fear association. We investigated the functional network supporting temporal associations using a task in which a 5-s interval separates the contextual CS from the US (CFC-5s). We quantified c-Fos expression in forty-nine brain regions of male rats following the CFC-5s training, used c-Fos correlations to generate functional networks, and analyzed them by graph theory. Control groups were trained in contextual fear conditioning, in which CS and US overlap. The CFC-5s training additionally activated subdivisions of the basolateral, lateral, and medial amygdala; prelimbic, infralimbic, perirhinal, postrhinal, and intermediate entorhinal cortices; ventral CA1 and subiculum. The CFC-5s network had increased amygdala centrality and higher amygdala internal and external connectivity with the retrosplenial cortex, thalamus, and hippocampus. Amygdala and thalamic nuclei were network hubs. Functional connectivity among these brain regions could support CS transient memories and their association. (AU)

• Processo FAPESP: 16/13027-2 - Rede neural subjacente à aquisição do condicionamento de medo em descontinuidade temporal e espacial: interações do córtex pré-límbico
• Beneficiário: Thays Brenner dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/04844-5 - Identificação de pequenos grupos neurais seletivamente ativados (neuronal ensembles) no condicionamento de medo com componentes temporais e contextuais
• Beneficiário: Thays Brenner dos Santos
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/25755-2 - Rede neural subjacente à aquisição do condicionamento de medo em descontinuidade temporal e espacial: interações do córtex pré-límbico
• Beneficiário: Maria Gabriela Menezes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/10837-7 - Rede funcional da memória contextual e temporal apresenta aumento da centralidade da amígdala e da conectividade da amígdala com o córtex retroesplenial, tálamo e hipocampo
• Beneficiário: Maria Gabriela Menezes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo