| Texto completo | |
| Autor(es): |
Rodrigues, Tiago
;
Ferraz, Leticia Silva
Número total de Autores: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | STAR PROTOCOLS; v. 2, n. 1, p. 12-pg., 2021-03-19. |
| Resumo | |
Cytosolic Ca2+ levels are maintained at low nanomolar concentrations, and disruption of Ca2+ homeostasis is associated with cell/tissue damage. Thus, methods have been developed to accurately assess cellular Ca2+ levels, each with intrinsic advantages and disadvantages. Here, we present in detail a ratio -metric fluorometric method for cytosolic Ca2+ measurement in cultured mela-noma cells using Fura 2-AM cell loading and fluorescence microscopy imaging. For complete details on the use and execution of this protocol, please refer to Esteves et al. (2020). (AU) | |
| Processo FAPESP: | 18/25747-5 - Alterações de morfologia e dinâmica mitocondriais no câncer: compreensão da biologia tumoral e prospecção de novos alvos terapêuticos |
| Beneficiário: | Tiago Rodrigues |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |