| Texto completo | |
| Autor(es): |
da Silva, Marluci Palazzolli
;
Rai, Rewa
;
Favaro-Trindade, Carmen S.
;
Nitin, Nitin
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FOOD BIOSCIENCE; v. 54, p. 10-pg., 2023-06-23. |
| Resumo | |
This study focused on developing novel compositions for the simultaneous delivery of probiotics and phenolic bioactives. These novel compositions were generated by biosorption of phenolic-rich plant extracts produced using jabuticaba peel, guaran & PRIME;a seed, and pure catechin in three probiotic strains using vacuum-assisted and passive incubations. Vacuum-assisted biosorption using jabuticaba peel in 25% of ethanol solution (JP25%) provided the maximum loading of phenolic compounds (PC), around 9 mg Gallic Acid Equivalent (GAE)/g of cells in less than 5 min without significantly influencing probiotic cell viability. In contrast, the total phenolic content of probiotic cells loaded with guaran & PRIME;a seed extract (GSE) and catechin solution ranged between 3.5 and 5 mg GAE/g of cells. The PC content of probiotic cells using vacuum-assisted biosorption was around 2-4 fold higher than that obtained via passive biosorption for 24 h. The biosorption of PCs in probiotic cells was also confirmed by multiphoton microscopy. The viability of freeze-dried cells at 25 degrees C up to 30 days loaded with plant extracts by vacuum assisted biosorption was 1-2 log CFU/g higher than cells passively loaded with plant extracts. Furthermore, PC release from Lacticaseibacillus cells ranged between 55% and 75% of biosorbed PCs during simulated gastrointestinal digestions. Overall, this study illustrates a novel approach to formulate combination of probiotics and PCs and their potential to deliver viable probiotics and bioactive PCs. (AU) | |
| Processo FAPESP: | 16/24895-5 - Coencapsulação dos extratos do guaraná (Paullinia cupana) e probióticos |
| Beneficiário: | Marluci Palazzolli da Silva Padilha |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 19/09757-3 - Encapsulação usando células como carreador: infusão de extratos vegetais |
| Beneficiário: | Marluci Palazzolli da Silva Padilha |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |