Early In Vivo Osteogenic and Inflammatory Response of 3D Printed Polycaprolactone/Carbon Nanotube/Hydroxyapatite/Tricalcium Phosphate Composite Scaffolds

Autor(es)

Nalesso, Paulo Roberto Lopes ; Vedovatto, Matheus ; Gregorio, Julia Eduarda Schneider ; Huang, Boyang ; Vyas, Cian ; Santamaria-Jr, Milton ; Bartolo, Paulo ; Caetano, Guilherme Ferreira

• Tipo de documento: Artigo Científico
• Fonte: POLYMERS; v. 15, n. 13, p. 14-pg., 2023-07-01.

Resumo

The development of advanced biomaterials and manufacturing processes to fabricate biologically and mechanically appropriate scaffolds for bone tissue is a significant challenge. Polycaprolactone (PCL) is a biocompatible and degradable polymer used in bone tissue engineering, but it lacks biofunctionalization. Bioceramics, such as hydroxyapatite (HA) and & beta; tricalcium phosphate (& beta;-TCP), which are similar chemically to native bone, can facilitate both osteointegration and osteoinduction whilst improving the biomechanics of a scaffold. Carbon nanotubes (CNTs) display exceptional electrical conductivity and mechanical properties. A major limitation is the understanding of how PCL-based scaffolds containing HA, TCP, and CNTs behave in vivo in a bone regeneration model. The objective of this study was to evaluate the use of three-dimensional (3D) printed PCL-based composite scaffolds containing CNTs, HA, and & beta;-TCP during the initial osteogenic and inflammatory response phase in a critical bone defect rat model. Gene expression related to early osteogenesis, the inflammatory phase, and tissue formation was evaluated using quantitative real-time PCR (RT-qPCR). Tissue formation and mineralization were assessed by histomorphometry. The CNT+HA/TCP group presented higher expression of osteogenic genes after seven days. The CNT+HA and CNT+TCP groups stimulated higher gene expression for tissue formation and mineralization, and pro- and anti-inflammatory genes after 14 and 30 days. Moreover, the CNT+TCP and CNT+HA/TCP groups showed higher gene expressions related to M1 macrophages. The association of CNTs with ceramics at 10wt% (CNT+HA/TCP) showed lower expressions of inflammatory genes and higher osteogenic, presenting a positive impact and balanced cell signaling for early bone formation. The association of CNTs with both ceramics promoted a minor inflammatory response and faster bone tissue formation. (AU)

• Processo FAPESP: 18/21167-4 - Emprego de scaffolds cerâmicos eletroativos e aplicação de corrente elétrica de baixa intensidade no reparo ósseo
• Beneficiário: Guilherme Ferreira Caetano
• Modalidade de apoio: Auxílio à Pesquisa - Regular