Combining IP3 affinity chromatography and bioinformatics reveals a novel protein-IP3 binding site on Plasmodium falciparum MDR1 transporter*

Autor(es)

Alves, Eduardo ; Nakaya, Helder ; Guimares, Euzebio ; Garcia, Celia R. S.

• Tipo de documento: Artigo Científico
• Fonte: CURRENT RESEARCH IN MICROBIAL SCIENCES; v. 4, p. 7-pg., 2022-12-19.

Resumo

Intracellular Ca2+ mobilization induced by second messenger IP3 controls many cellular events in most of the eukaryotic groups. Despite the increasing evidence of IP3-induced Ca2+ in apicomplexan parasites like Plasmodium, responsible for malaria infection, no protein with potential function as an IP3-receptor has been identified. The use of bioinformatic analyses based on previously known sequences of IP3-receptor failed to identify potential IP3-receptor candidates in any Apicomplexa. In this work, we combine the biochemical approach of an IP3 affinity chromatography column with bioinformatic meta-analyses to identify potential vital membrane proteins that present binding with IP3 in Plasmodium falciparum. Our analyses reveal that PF3D7_0523000, a gene that codes a transport protein associated with multidrug resistance as a potential target for IP3. This work provides a new insight for probing potential candidates for IP3-receptor in Apicomplexa. (AU)

• Processo FAPESP: 17/08684-7 - Decodificar aspectos da biologia celular e molecular do Plasmodium como uma ferramenta para desenvolver novos antimaláricos
• Beneficiário: Célia Regina da Silva Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/14933-2 - Biologia integrativa aplicada à saúde humana
• Beneficiário: Helder Takashi Imoto Nakaya
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 18/07177-7 - EMU concedido no processo 2011/51295-5: image system
• Beneficiário: Célia Regina da Silva Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários