Flowing through Gastrointestinal Barriers with Model Nanoparticles: From Complex Fluids to Model Human Intestinal Epithelium Permeation

Autor(es)

Ribeiro, Iris Renata Sousa ; da Silva, Raquel Frenedoso ; Rabelo, Renata Santos ; Marin, Talita Miguel ; Bettini, Jefferson ; Cardoso, Mateus Borba

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 15, n. 30, p. 11-pg., 2023-07-19.

Resumo

Most nanomaterial-based medicines are intravenously appliedsinceoral administration comprises challenging-related biological obstacles,such as interactions with distinct digestive fluids and their transportthrough the intestinal barrier. Moreover, there is a lack of nanoparticle-basedstudies that faithfully consider the above-cited obstacles and boostoral-administered nanomedicines' rational design. In this study,the physicochemical stability of fluorescent model silica nanoparticles(f-SiO(2)NPs) passing through all simulated gastrointestinalfluids (salivary, gastric, and intestinal) and their absorption andtransport across a model human intestinal epithelium barrier are investigated.An aggregation/disaggregation f-SiO(2)NPs process is identified,although these particles remain chemically and physically stable afterexposure to digestive fluids. Further, fine imaging of f-SiO(2)NPs through the absorption and transport across the human intestinalepithelium indicates that nanoparticle transport is time-dependent.The above-presented protocol shows tremendous potential for decipheringfundamental gastrointestinal nanoparticles' evolution and cancontribute to rational oral administration-based nanomedicine design. (AU)

• Processo FAPESP: 21/12071-6 - Arquitetando coloides via interações supramoleculares: de fundamentos a aplicações
• Beneficiário: Watson Loh
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/25406-5 - Organizando a matéria: colóides formados por associação de surfactantes, polímeros e nanopartículas
• Beneficiário: Watson Loh
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/21318-0 - Direcionamento de nanopartículas funcionalizadas em sistemas microfluídicos contendo múltiplos organoides
• Beneficiário: Iris Renata Sousa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado