| Texto completo | |
| Autor(es): |
Alves da Silva Pimentel, Bruna Natalia
;
De Annunzio, Sarah Raquel
;
Assis, Marcelo
;
Barbugli, Paula Aboud
;
Longo, Elson
;
Vergani, Carlos Eduardo
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 11, p. 13-pg., 2023-07-20. |
| Resumo | |
Silver tungstate (alpha-Ag2WO4), silver molybdate (beta-Ag2MoO4), and silver vanadate (alpha-AgVO3) microcrystals have shown interesting antimicrobial properties. However, their biocompatibility is not yet fully understood. Cytotoxicity and the inflammatory response of silver-containing microcrystals were analyzed in THP-1 and THP-1 differentiated as macrophage-like cells, with the alamarBlue (TM) assay, flow cytometry, confocal microscopy, and ELISA. The present investigation also evaluated redox signaling and the production of cytokines (TNF alpha, IL-1 beta, IL-6, and IL-8) and matrix metalloproteinases (MMP-8 and -9). The results showed that alpha-AgVO3 (3.9 mu g/mL) did not affect cell viability (p > 0.05). alpha-Ag2WO4 (7.81 mu g/mL), beta-Ag2MoO4 (15.62 mu g/mL), and alpha-AgVO3 (15.62 mu g/mL) slightly decreased cell viability (p <= 0.003). All silver-containing microcrystals induced the production of O-2(-) and this effect was mitigated by Reactive Oxygen Species (ROS) scavenger and N-acetylcysteine (NAC). TNF alpha, IL-6 and IL-1 beta were not detected in THP-1 cells, while their production was either lower (p <= 0.0321) or similar to the control group (p >= 0.1048) for macrophage-like cells. The production of IL-8 by both cellular phenotypes was similar to the control group (p >= 0.3570). The release of MMP-8 was not detected in any condition in THP-1 cells. Although MMP-9 was released by THP-1 cells exposed to alpha-AgVO3 (3.9 mu g/mL), no significant difference was found with control (p = 0.7). Regarding macrophage-like cells, the release of MMP-8 and -9 decreased in the presence of all microcrystals (p <= 0.010). Overall, the present work shows a promising biocompatibility profile of, alpha-Ag2WO4, beta-Ag2MoO4, and alpha-AgVO3 microcrystals. (AU) | |
| Processo FAPESP: | 18/01677-8 - Biocompatibilidade dos microcristais ±-Ag2WO4, ²-Ag2MoO4 e ±-AgVO3: avaliação de citocinas pró-inflamatórias e metaloproteinases da matriz |
| Beneficiário: | Bruna Natalia Alves da Silva Pimentel Lira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 22/08487-5 - Propriedades antimicrobianas e biocompatibilidade de titânio e zircônia revestidos com ±-Ag2WO4 irradiado com feixe de elétrons |
| Beneficiário: | Sarah Raquel de Annunzio |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |