| Texto completo | |
| Autor(es): |
Arantes, Guilherme M.
Número total de Autores: 1
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| Tipo de documento: | Artigo Científico |
| Fonte: | Biochemical Journal; v. 399, p. 8-pg., 2006-10-15. |
| Resumo | |
PTPs (protein tyrosine phosphatases) are fundamental enzymes for cell signalling and have been linked to the pathogenesis of several diseases, including cancer. Hence, PTPs are potential drug targets and inhibitors have been designed as possible therapeutic agents for Type II diabetes and obesity. However, a complete understanding of the detailed catalytic mechanism in PTPs is still lacking. Free-energy profiles, obtained by computer simulations of catalysis by a dual-specificity PTP, are shown in the present study and are used to shed light on the catalytic mechanism. A highly accurate hybrid potential of quantum mechanics/molecular mechanics calibrated specifically for PTP reactions was used. Reactions of alkyl and aryl substrates, with different protonation states and PTP active-site mutations, were simulated. Calculated reaction barriers agree well with experimental rate measurements. Results show the PTP substrate reacts as a bi-anion, with an ionized nucleophile. This protonation state has been a matter of debate in the literature. The inactivity of Cys -> Ser active-site mutants is also not fully understood. It is shown that mutants are inactive because the serine nucleophile is protonated. Results also clarify the interpretation of experimental data, particularly kinetic isotope effects. The simulated mechanisms presented here are better examples of the catalysis carried out by PTPs. (AU) | |
| Processo FAPESP: | 99/07688-9 - Estruturação e reatividade em meios homogêneo e microheterogêneo |
| Beneficiário: | Luis Gustavo Dias |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |