| Texto completo | |
| Autor(es): |
Camargo, Andre F.
;
Chioda, Marina M.
;
Rodrigues, Ana P. C.
;
Garcia, Geovana S.
;
McKinney, Emily A.
;
Jacobs, Howard T.
;
Oliveira, Marcos T.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Cell Biology International; v. 42, n. 6, p. 6-pg., 2018-06-01. |
| Resumo | |
The mitochondrial respiratory chain in vertebrates and arthropods is different from that of most other eukaryotes because they lack alternative enzymes that provide electron transfer pathways additional to the oxidative phosphorylation (OXPHOS) system. However, the use of diverse experimental models, such as human cells in culture, Drosophila melanogaster and the mouse, has demonstrated that the transgenic expression of these alternative enzymes can impact positively many phenotypes associated with human mitochondrial and other cellular dysfunction, including those typically presented in complex IV deficiencies, Parkinson's, and Alzheimer's. In addition, these enzymes have recently provided extremely valuable data on how, when, and where reactive oxygen species, considered by many as by-products of OXPHOS, can contribute to animal longevity. It has also been shown that the expression of the alternative enzymes is thermogenic in cultured cells, causes reproductive defects in flies, and enhances the deleterious phenotype of some mitochondrial disease models. Therefore, all the reported beneficial effects must be considered with caution, as these enzymes have been proposed to be deployed in putative gene therapies to treat human diseases. Here, we present a brief review of the scientific data accumulated over the past decade that show the benefits and the risks of introducing alternative branches of the electron transport into mammalian and insect mitochondria, and we provide a perspective on the future of this research field. (AU) | |
| Processo FAPESP: | 17/03806-7 - Avaliação do potencial termogênico da oxidase alternativa mitocondrial expressa em Drosophila melanogaster |
| Beneficiário: | Geovana Siqueira Garcia |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 14/02253-6 - Investigando as alterações metabólicas causadas pela expressão transgênica da oxidase alternativa mitocondrial de Ciona intestinalis em Drosophila melanogaster |
| Beneficiário: | Marcos Túlio de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 17/02813-0 - Proteômica mitocondrial de Drosophila melanogaster expressando a oxidase alternativa sob diferentes condições dietéticas |
| Beneficiário: | Marina Minari Chioda |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/14547-7 - Efeitos da expressão da AOX em mutantes da helicase mitocondrial de Drosophila melanogaster |
| Beneficiário: | Ana Paula Campos Rodrigues |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |