Diagnostic and vaccine potential of Zika virus envelope protein (E) derivates produced in bacterial and insect cells

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Lunardelli, Victoria Alves Santos ; Almeida, Bianca da Silva ; Apostolico, Juliana de Souza ; Rezende, Thais ; Yamamoto, Marcio Massao ; Pereira, Samuel Santos ; Bueno, Maria Fernanda Campagnari ; Pereira, Lennon Ramos ; Carvalho, Karina Inacio ; Slhessarenko, Renata Dezengrini ; Ferreira, Luis Carlos de Souza ; Boscardin, Silvia Beatriz ; Rosa, Daniela Santoro

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 14, p. 14-pg., 2023-03-16.

Resumo

IntroductionIn the present study we evaluated the features of different recombinant forms of Zika virus (ZIKV) proteins produced in either bacterial (Eschericha coli) or insect cells (Drosophila melanogaster). The ZIKV-envelope glycoprotein (E-ZIKV) is responsible for virus entry into host cells, is the main target of neutralizing antibodies and has been used as a target antigen either for serological tests or for the development of subunit vaccines. The E-ZIKV is composed of three structural and functional domains (EDI, EDII, and EDIII), which share extensive sequence conservation with the corresponding counterparts expressed by other flaviviruses, particularly the different dengue virus (DENV) subtypes. MethodsIn this study, we carried out a systematic comparison of the antigenicity and immunogenicity of recombinant EZIKV, EDI/IIZIKV and EDIIIZIKV produced in E. coli BL21 and Drosophila S2 cells. For the antigenicity analysis we collected 88 serum samples from ZIKV-infected participants and 57 serum samples from DENV-infected. For immunogenicity, C57BL/6 mice were immunized with two doses of EZIKV, EDI/IIZIKV and EDIIIZIKV produced in E. coli BL21 and Drosophila S2 cells to evaluate humoral and cellular immune response. In addition, AG129 mice were immunized with EZIKV and then challenge with ZIKV. ResultsTesting of samples collected from ZIKV-infected and DENV-infected participants demonstrated that the EZIKV and EDIIIZIKV produced in BL21 cells presented better sensitivity and specificity compared to proteins produced in S2 cells. In vivo analyses were carried out with C57BL/6 mice and the results indicated that, despite similar immunogenicity, antigens produced in S2 cells, particularly EZIKV and EDIIIZIKV, induced higher ZIKV-neutralizing antibody levels in vaccinated mice. In addition, immunization with EZIKV expressed in S2 cells delayed the onset of symptoms and increased survival rates in immunocompromised mice. All recombinant antigens, either produced in bacteria or insect cells, induced antigen-specific CD4+ and CD8+ T cell responses. ConclusionIn conclusion, the present study highlights the differences in antigenicity and immunogenicity of recombinant ZIKV antigens produced in two heterologous protein expression systems. (AU)

• Processo FAPESP: 21/13004-0 - Desenvolvimento de vacinas de subunidade contra o SARS-CoV-2 baseadas no domínio de ligação ao receptor (RBD)
• Beneficiário: Daniela Santoro Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/17471-7 - Antigenicidade e imunogenicidade de proteínas recombinantes do envelope viral do Zika vírus
• Beneficiário: Daniela Santoro Rosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular