Identification of Clinical Trichosporon asteroides Strains by MALDI-TOF Mass Spectrometry: Evaluation of the Bruker Daltonics Commercial System and an In-House Developed Library

Autor(es)

Francisco, Elaine Cristina ; Ebbing, Mexx ; Colombo, Arnaldo Lopes ; Hagen, Ferry ; Trichosporon Brazilian Network

• Tipo de documento: Artigo Científico
• Fonte: Mycopathologia; v. 188, n. 3, p. 7-pg., 2023-04-15.

Resumo

Trichosporon asteroides is an emerging yeast-like pathogen commonly misidentified by commercial biochemical identification systems. We evaluated the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the identification of 21 clinical T. asteroides strains using the Bruker Daltonics database (BDAL) and an in-house developed library. Mass spectra were obtained by the FlexControl system v.3.4, and characterizations were performed in the Biotyper BDAL database v.4.1 and the developed in-house library. Species identification for T. asteroides failed as all 21 strains were misidentified as T. japonicum (log-scores 1.89-2.19). Extending the existing database was crucial to achieving 100% correct species-level identification and accurate distinction between species. Our results indicate that the commercial BDAL database has no discriminatory power to distinguish between T. japonicum and T. asteroides. Whereas improvement of the current BDAL database is pending, we strongly advise system users not to exclude the possibility of the failure to report T. asteroides. (AU)

• Processo FAPESP: 19/24960-0 - Parceria internacional para avaliação da heterogeneidade genética de isolados clínicos de Trichosporon sp. e seu impacto na taxonomia, biologia e identificação de espécies
• Beneficiário: Elaine Cristina Francisco Del Ciello
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/14097-0 - Parceira internacional para a caracterização da heterogeneidade genética de isolados clínicos de Trichosporon spp. e seu impacto na taxonomia, biologia e identificação de espécies
• Beneficiário: Elaine Cristina Francisco Del Ciello
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado