Identification of biomarkers in diabetic nails by Raman spectroscopy

Autor(es)

Veras, Juscelia Maria de Moura Feitosa ; Coelho, Lennara de Siqueira ; Neto, Lazaro Pinto Medeiros ; de Almeida, Ritiane Modesto ; da Silva, Gustavo Carlos ; de Santana, Felipe Bachion ; Garcia, Livia Assis ; Martin, Airton Abrahao ; Favero, Priscila Pereira

• Tipo de documento: Artigo Científico
• Fonte: Clinica Chimica Acta; v. 544, p. 6-pg., 2023-04-28.

Resumo

Background: The growth of Diabetes Mellitus (DM) is a serious public health issue which is more prevalent in developing countries. The main problems related to DM are the gradual changes in the structural and functional integrity of tissues caused by hyperglycemia, which calls for early diagnosis and periodic monitoring exams. Recent studies suggest that the quality of the nail plate has great potential to assess the secondary complications of DM. Hence, this study aimed to determine the biochemical characteristics of the nails of individuals with DM2 by Raman confocal spectroscopy (CRS). Methods: We collected fragments from the distal region of the fingernails of 30 healthy volunteers and 30 volunteers with DM2. The samples were analyzed by CRS (Xplora - Horiba) coupled to a 785 nm laser. Results: Alterations in different biochemical components, such as proteins, lipids, amino acids, and final agents of advanced glycation, and alterations in the disulfide bridges, which are important in stabilizing keratin in nails were identified. Conclusion: The spectral signatures and new DM2 markers in nails were identified. Therefore, the possibility of acquiring biochemical information by evaluating the nails of diabetics, a simple and easily acquired material associated with the CRS technique, may allow health complications to be detected quickly. (AU)

• Processo FAPESP: 20/00928-7 - Desenvolvimento de processo in vivo e não invasivo para a quantificação de ácido hialurônico na pele humana
• Beneficiário: Lázaro Pinto Medeiros Neto
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE