Impacts of maternal separation stress on ethanol-related responses, anxiety-and depressive-like behaviors in adolescent mice

Autor(es)

Favoretto, C. A. ; Bertagna, N. B. ; Righi, T. ; Rodolpho, B. T. ; Anjos-Santos, A. ; Silva, F. B. R. ; Bianchi, P. C. ; Cruz, F. C.

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience Letters; v. 809, p. 12-pg., 2023-05-23.

Resumo

The present work evaluated the consequences of chronic maternal separation (MS), an animal model of early-life stress, on ethanol intake and striatal Fos expression induced by ethanol consumption. Furthermore, we analyzed MS impacts on anxiety- and depressive-like behaviors and on locomotor and plasma corticosterone responses to intraperitoneal treatment with ethanol in adolescent mice. For that, male and female C57BL/6J mice were exposed or not to MS stress, for 3 h per day, from postnatal day (PND) 1 to 14, and submitted to behavioral tests from PND 28. In Experiment 1, MS and control groups of mice were submitted to an involuntary ethanol intake protocol, and striatal Fos expression following ethanol exposure was analyzed. In Experiment 2, mice behavior was assessed in elevated plus-maze, sucrose splash, saccharin preference, and open field tests. Locomotor and plasma corticosterone responses induced by a systemic dose of ethanol (1.75 g/kg) were also evaluated. Our results demonstrated that MS increased ethanol intake only in an acute manner and did not impact ethanolinduced Fos expression in the dorsal striatum and nucleus accumbens (NAc) core and shell subregions. MS did not change the parameters analyzed during elevated plus-maze, sucrose splash, preference for saccharin, and open field tests. MS did not affect locomotor activity following ethanol injection nor plasma corticosterone response to the drug. Thus, our data showed that MS transiently increased ethanol intake. However, early-life stress did not impact Fos, locomotor, or plasma corticosterone responses to the drug. In addition, MS did not affect anxiety- and depressive-like behaviors in adolescent mice. (AU)

• Processo FAPESP: 19/24073-3 - Papel dos receptores canabinóides CB1 na modulação de circuitos neurais envolvidos na interação entre o estresse de separação materna e consumo de etanol em camundongos
• Beneficiário: Cristiane Aparecida Favoretto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/15505-4 - Estudo neurobiológico da recaída ao uso de cocaína e crack: identificação de plasticidades em neuronal ensembles que armazenam memórias relacionadas com a adição de drogas
• Beneficiário: Fabio Cardoso Cruz
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2