Capsicum chinense var. BRS Moema: chemical characterization by HPLC-ESI-MS/MS and antiproliferative screening

Autor(es)

de Aguiar, Ana Carolina ; Pereira, Gustavo Araujo ; Ribeiro, Claudia Silva da Costa ; Eberlin, Marcos Nogueira ; Soares, Lana Pereira ; Ruiz, Ana Lucia Tasca Gois ; Pastore, Glaucia Maria ; Martinez, Julian

• Tipo de documento: Artigo Científico
• Fonte: FOOD & FUNCTION; v. 14, n. 14, p. 11-pg., 2023-06-19.

Resumo

Capsiate and phenolics present in the free, esterified, glycosylated, and insoluble-bound forms of BRS Moema peppers were characterized and quantified using UHPLC-ESI-MS/MS. Additionally, the in vitro antiproliferative activity of BRS Moema extract was evaluated. The peppers showed considerable quantities of capsiate and phenolic compounds. Esterified phenolics were the main fraction, followed by the insoluble-bound fraction, indicating that relying solely on the extraction of soluble phenolics may underestimate the total phenolic content. Among the fourteen phenolics identified in extract fractions, gallic acid was the major constituent. Phenolic fractions displayed high antioxidant capacity by TEAC and ORAC assays. Nevertheless, the correlation between phenolic compounds and antioxidant activity suggested that other bioactive or phenolic compounds may contribute to the overall phenolic compounds and antioxidant capacity of the obtained fractions. Concerning the antiproliferative activity, the extract did not exhibit any effect on cell proliferation within the evaluated concentration range. These findings indicated that BRS Moema peppers can serve as a rich source of phenolic compounds. Therefore, fully utilizing them could bring advantages to the food and pharmaceutical industries, as well as to consumers and producers. (AU)

• Processo FAPESP: 15/18119-0 - Emulsificação por ultrassom de extratos de pimentas biquinho (Capsicum chinense) obtidos por extração supercrítica como alternativa para o aumento da bioatividade
• Beneficiário: Ana Carolina de Aguiar
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/23950-7 - Avaliação da atividade (anti)mutagênica in vitro de um alcaloide aporfínico e seus intermediários de síntese
• Beneficiário: Tamires Carolina Sedano
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/16903-0 - Fabricação de nanoemulsões comestíveis de oleoresina de pimenta Capsicum chinense empregando a técnica de microfluidização
• Beneficiário: Ana Carolina de Aguiar
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 11/01114-4 - Atividade antiproliferativa e ação hormonal/anti-hormonal de extratos e frações enriquecidas de algumas espécies de Mentha (Lamiaceae)
• Beneficiário: Ana Lúcia Tasca Gois Ruiz
• Modalidade de apoio: Auxílio à Pesquisa - Regular