Antimicrobial Formulation of a Bacterial Nanocellulose/Propolis-Containing Photosensitizer for Biomedical Applications

Autor(es)

Goncalves, Isabella Salgado ; Lima, Lais Roncalho ; Berretta, Andresa Aparecida ; Amorim, Nathaly Alcazar ; Pratavieira, Sebastiao ; Correa, Thaila Quatrini ; Resende Nogueira, Flavia Aparecida ; Barud, Hernane Silva

• Tipo de documento: Artigo Científico
• Fonte: POLYMERS; v. 15, n. 4, p. 20-pg., 2023-02-01.

Resumo

With the aim of contributing to the development of more efficient materials for wound care, new topical formulations based on bacterial nanocellulose (BNC) hydrogels containing propolis were produced. Characterizations confirmed the incorporation of propolis into the BNC matrix, maintaining its structure and properties. Rheological analysis confirmed that the hydrogels showed thixotropic behavior appropriate for topical application. Chromatographic profiles showed sustained release of propolis biomarkers for at least 20 h. The formulations did not present mutagenicity. For application in photodynamic inactivation (PDI), BNC/propolis hydrogels were prepared with the photosensitizers methylene blue (MB). Spectroscopy and confocal fluorescence microscopy confirmed the interaction of MB and propolis in BNC hydrogels, as well as the formation of a new composite material. In the antibacterial assays, formulations containing MB and propolis significantly reduced Staphylococcus aureus growth. In the presence of light, BNC/MB hydrogels completely inhibited the microorganism. Therefore, the results suggest potential materials for the prevention or treatment of Staphylococcus aureus infections in wounds. (AU)

• Processo FAPESP: 13/07793-6 - CEPIV - Centro de Ensino, Pesquisa e Inovação em Vidros
• Beneficiário: Edgar Dutra Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 17/16278-9 - Estudo do potencial genotoxicológico e da permeabilidade em células Caco-2 de complexos metálicos com promissoras atividades biológicas
• Beneficiário: Flávia Aparecida Resende Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/25512-8 - Plataforma para Cultura de Células Baseada em Biocelulose
• Beneficiário: Hernane da Silva Barud
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários