A Gelatin Methacrylate-Based Hydrogel as a Potential Bioink for 3D Bioprinting and Neuronal Differentiation

Autor(es)

Cruz, Elisa Marozzi ; Machado, Lucas Simoes ; Zamproni, Laura Nicoleti ; Bim, Larissa Valdemarin ; Ferreira, Paula Scanavez ; Pinto, Leonardo Alves ; Pessan, Luiz Antonio ; Backes, Eduardo Henrique ; Porcionatto, Marimelia Aparecida

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 15, n. 2, p. 22-pg., 2023-02-01.

Resumo

Neuronal loss is the ultimate pathophysiologic event in central nervous system (CNS) diseases and replacing these neurons is one of the most significant challenges in regenerative medicine. Providing a suitable microenvironment for new neuron engraftment, proliferation, and synapse formation is a primary goal for 3D bioprinting. Among the various biomaterials, gelatin methacrylate (GelMA) stands out due to its Arg-Gly-Asp (RGD) domains, which assure its biocompatibility and degradation under physiological conditions. This work aimed to produce different GelMA-based bioink compositions, verify their mechanical and biological properties, and evaluate their ability to support neurogenesis. We evaluated four different GelMA-based bioink compositions; however, when it came to their biological properties, incorporating extracellular matrix components, such as Geltrex (TM), was essential to ensure human neuroprogenitor cell viability. Finally, Geltrex (TM): 8% GelMA (1:1) bioink efficiently maintained human neuroprogenitor cell stemness and supported neuronal differentiation. Interestingly, this bioink composition provides a suitable environment for murine astrocytes to de-differentiate into neural stem cells and give rise to MAP2-positive cells. (AU)

• Processo FAPESP: 18/12605-8 - Desenvolvimento de microplataformas brain-on-a-chip para modelagem do sistema nervoso central in vitro
• Beneficiário: Marimélia Aparecida Porcionatto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/12454-0 - Bioimpressão 3D de células-tronco neurais derivadas de astrócitos reativos
• Beneficiário: Elisa Marozzi Cruz
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/11374-2 - Investigação dos efeitos da infecção de neurônios e astrócitos por SARS-CoV-2 na neuroinflamação utilizando tecido neural bioimpresso
• Beneficiário: Eduardo Henrique Backes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/27415-2 - Scaffolds bioativos com modificação superficial por plasma
• Beneficiário: Luiz Antonio Pessan
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/14327-8 - Hemorragia subaracnóide em um chip
• Beneficiário: Laura Nicoleti Zamproni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado